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ارزیابی چرخه سلولی سلولهای سرطان انسانی به وسیله فلوسایتومتری به دنبال تاثیر دی متیل سولفون

جعفری, ناصر ، بهلولی, شهاب (1390) ارزیابی چرخه سلولی سلولهای سرطان انسانی به وسیله فلوسایتومتری به دنبال تاثیر دی متیل سولفون. در: دوازدهمين کنگره بيوشيمی ايران و چهارمين کنگره بين المللی بيوشيمی و بيولوژی مولکولی, 15 تا 18 شهريور 1390, ایران - مشهد.

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آدرس اینترنتی رسمی : http://www.sciencedirect.com/science/article/pii/S...


عنوان انگليسي

Flow cytometric analysis of the effect of dimethylsulfone on cell cycle arrest in human cancer cell lines

خلاصه انگلیسی

Introduction: Dimethylsulfone (MSM) is a dietary supplement to support healthy body. It has been suggested that MSM has a chemopreventive mechanism that affects the interaction of tumor cells with the host immune response. Considering preventive effects of this substance on tumor onset and non-toxic to healthy body we investigated in vitro effects of dimethylsulfone on cell cycle distribution in cancer cell lines. Materials and methods: Cells were seeded in 25 cm2 flasks at a density of 1×106 cells/flask. Cells were treated with MSM for 24 h at a range of concentration. After treatment, the DNA content and cellcycle distribution were determined by flow cytometry. Briefly, cells were trypsinized and harvested, then cells fixed with 70% ice ethanol. Fixed cells were centrifuged and washed with cold PBS, and then stained with DAPI 10 μg/ml. The stained cells were then transferred to flow tubes by passing through a nylon mesh with a pore size of 30 μm. Results: The percentage of cells in G1, S and G2/M phase was calculated using Partec FloMax software. We observed a dosedependent effect of MSM on the cell cycle. After 24 h of MSM treatment at different concentrations, cells in the G2/M population increased compared to controls. The effect of MSM on cancer cells appears to be dose-dependent. The higher the dosage, the greater the G2/M population increase. Conclusion: The results showed that MSM is able to induce a G2 M cell cycle arrest in gastrointestinal cancer cell lines

نوع سند :موضوع کنفرانس یا کارگاه (سخنرانی کلاسی)
زبان سند : انگلیسی
نویسنده مسئول :ناصر جعفری
نویسنده :شهاب بهلولی
کلید واژه ها (انگلیسی):Dimethylsulfone, Dietary supplement, Cell cycle, Cancer cell lines
موضوعات :QU بیوشیمی
QV فارماکولوژی
بخش های دانشگاهی :دانشكده پزشكي > گروه علوم پایه > بخش بیوشیمی
دانشكده پزشكي > گروه علوم پایه > بخش فارماکولوژی
کد شناسایی :2667
ارائه شده توسط : آقای ناصر جعفری
ارائه شده در تاریخ :07 مهر 1390 07:43
آخرین تغییر :28 بهمن 1391 09:36

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