title

پیوند همزمان سلولهای بنیادی استرومای مغز قرمز استخوان با سلولهای شوان در مدل ضایعه نخاعی Contusion، آلودینای مکانیکی را کاهش می دهد

پورحیدر, باقر ، جغتایی, محمد تقی ، بختیاری, مهرداد ، مهدی زاده, مهدی ، یکتا, زهرا ، نجف زاده, نوروز (1390) پیوند همزمان سلولهای بنیادی استرومای مغز قرمز استخوان با سلولهای شوان در مدل ضایعه نخاعی Contusion، آلودینای مکانیکی را کاهش می دهد. Yakhteh Medical Journal ــ 13 (4). ص.ص.213-222. شاپا 1561-4921

[img]
پیش نمایش
متنی - نسخه چاپ شده
907kB

آدرس اینترنتی رسمی : http://www.celljournal.org/components4.php?rQV==wH...


عنوان انگليسي

CO- TRANSPLANTATION OF BONE MARROW STROMAL CELLS WITH SCHWANN CELLS EVOKES MECHANICAL ALLODYNIA IN THE CONTUSION MODEL OF SPINAL CORD INJURY IN RATS

خلاصه انگلیسی

Objective: Several studies have shown that, although transplantation of neural stem cells into the contusion model of spinal cord injury (SCI) promotes locomotor function and improves functional recovery, it induces a painful response, Allodynia. Different studies indicate that bone marrow stromal cells (BMSCs) and Schwann cells (SCs) can improve locomotor recovery when transplanted into the injured rat spinal cord. Since these cells are commonly used in cell therapy, we investigated whether co-transplantation of these cells leads to the development of Allodynia. Materials and Methods: In this experimental research, the contusion model of SCI was induced by laminectomy at the T8-T9 level of the spinal cord in adult female wistar rats (n=40) weighting (250-300g) using the New York University Device. BMSCs and SCs were cultured and prelabeled with 5-bromo-2-deoxyuridine (BrdU) and 1, 1' -dioctadecyl-3, 3, 3', 3' -tetramethylindocarbocyanine perchlorate (DiI) respectively. The rats were divided into five groups of 8 including: a control group (laminectomy only), three experimental groups (BMSC, SC and Co-transplant) and a sham group. The experimental groups received BMSCs, SCs, and BMSCs and SCs respectively by intraspinal injection 7 days after injury and the sham group received serum only. Locomotion was assessed using Basso, Beattie and Bresnahan (BBB) test and Allodynia by the withdrawal threshold test using Von Frey Filaments at 1, 7, 14, 21, 28, 35, 42, 49 and 56 days after SCI. The statistical comparisons between groups were carried out by using repeated measures analysis of variances (ANOVA). Results: Significant differences were observed in BBB scores in the Co- transplant group compared to the BMSC and SC groups (p<0.05). There were also significant differences in the withdrawal threshold means between animals in the sham group and the BMSC, SC and the Co-transplant groups (p<0.05). BBB scores and withdrawal threshold means showed that co-transplation improved functioning but greater Allodynia compared to the other experimental groups. Conclusion: The present study has shown that, although transplantation of BMSCs, SCs and a combination of these cells into the injured rat spinal cord can improve functional recovery, it leads to the development of mechanical Allodynia. This finding indicates that strategies to reduce Allodynia in cell transplantation studies are required.

نوع سند :مقاله
زبان سند : انگلیسی
نویسنده مسئول :باقر پورحیدر
اطلاعات اضافی :Impact Factor : 0.364 Indexed in: 1-Thomson Reuters (ISI)3.Biosis Preview Index Medicus for the Eastern Mediterranean Region(IMEMR), Index Copernicus International, Cambridge Scientific Abstracts EMBASE , Scopus , CINAHL Database 4. Google Scholar , Chemical Abstracts Service ,Proquest , National Library of Medicine (NLM) Directory of Open Access Journals (DOAJ) , Scientific Information Database (SID), Iranmedex , Regional Information Center for Sciences and Technology (RICeST) Islamic World Science Citation Center (ISC) , Magiran
کلید واژه ها:پیوند سلولی، سلولهای بنیادی،آسیب های نخاعی، آلودینا
کلید واژه ها (انگلیسی):CELL TRANSPLANTATION, STEM CELL, SPINAL CORD INJURIES, ALLODYNIA
موضوعات :QS آناتومی انسان
WL سیستم عصبی
بخش های دانشگاهی :دانشكده پزشكي > گروه علوم پایه > بخش علوم تشريح
کد شناسایی :3296
ارائه شده توسط : دکتر نوروز نجف زاده
ارائه شده در تاریخ :17 اردبهشت 1391 08:05
آخرین تغییر :07 مهر 1393 06:17

فقط پرسنل کتابخانه صفحه کنترل اسناد

Document Downloads

More statistics for this item...