مهار آنزیم امین اکسیداز جوانه نخود توسط تترااتیلن پنتامین

طلایی, سونیا ، اسدی, اسد ، امانی, مجتبی (1391) مهار آنزیم امین اکسیداز جوانه نخود توسط تترااتیلن پنتامین. Molecular Biology Research Communications (MBRC) ــ 1 (1). ص.ص.27-32. شاپا 2322-181X

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عنوان انگليسي

Inhibition of chickpea seedling copper amine oxidases by tetraethylenepentamine

خلاصه انگلیسی

Copper amine oxidases are important enzymes, which contribute to the regulation of monoand polyamine levels. Each monomer contains one Cu(II) ion and 2,4,5-trihydroxyphenylalanine (TPQ) as cofactors. They catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. The mechanism is followed by the transfer of two electrons to molecular oxygen which is reduced to hydrogen peroxide. Inhibitors are important tools in the study of catalytic properties of copper amine oxidases and they also have a wide application in physiological research. In this study, purification of the chickpea seedling amine oxidase, was done via salting out by ammonium sulfate and dialysis, followed by DEAE-cellulose column chromatography. By using the Lineweaver - Burk plot, the Km and Vm of the enzyme were found to be 3.3 mM and 0.95 mmol/min/mg, respectively. In this study, the interaction of chickpea diamino oxidase with tetraethylene- pentamine was studied. Analysis of kinetic data indicated that tetraethylenepentamine (with Ki=0.1 mM) inhibits the enzyme by linear mixed inhibitory effect.

نوع سند :مقاله
زبان سند : انگلیسی
نویسنده اول :سونیا طلایی
نویسنده مسئول :اسد اسدی
نویسنده :مجتبی امانی
اطلاعات اضافی :Indexed: Index Copernicus ,JournalTOCs , Electronic Journals Library (EZB) , Cancer Research Library Online (CaRLO) ,SHERPA/RoMEO ,ResearchBib ,Virtual Library of Biology (vifabio), Electronic Journals (e.journals) Electronic Journals (WZB), Public Knowledge Project (PKP) NewJour ,Free Journals Act ,Academic Keys ,Undergoing in other international databases ,
کلید واژه ها (انگلیسی):Chickpea; Copper-containing amine oxidases; Tetraethylenepentamine; Linear mixed
موضوعات :QU بیوشیمی
بخش های دانشگاهی :دانشكده پزشكي > گروه علوم پایه > بخش بیوفیزیک
کد شناسایی :3760
ارائه شده توسط : دکتر مجتبی امانی
ارائه شده در تاریخ :06 آذر 1391 13:52
آخرین تغییر :20 فروردین 1393 12:37

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