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اثرات محافظتی ان-استیل سیستئین بر استرس اکسیداتیو القا شده با مالاتیون در سلولهای کبدی جداشده از موش صحرائی

مصطفی لو, سارا ، عبدالهی, محمد ، اقبال, محمد علی ، سعیدی کوزه کنانی, نازلی (1390) اثرات محافظتی ان-استیل سیستئین بر استرس اکسیداتیو القا شده با مالاتیون در سلولهای کبدی جداشده از موش صحرائی. Advanced pharmaceutical bulletin ــ 2 (1). ص.ص.79-88. شاپا 2228-5881

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آدرس اینترنتی رسمی : http://apb.tbzmed.ac.ir/AllIssues/Volume22012/2179...


عنوان انگليسی

Protective effect of NAC against malathion-induced oxidative stress in freshly isolated rat hepatocytes

خلاصه انگلیسی

PURPOSE: Induction of oxidative stress by Organophosphate compounds (OPs) has been previously reported. In the present work, the mechanism of protective effects of N-acetylcysteine as a glutathion (GSH) prodrug against malathion-induced cell toxicity was investigated. In this work, freshly isolated rat hepatocytes were used to determine the effect of NAC on malathion-induced cytotoxicity, formation of reactive oxygen species (ROS) and mitochondrial dysfunction. METHODS: Rat hepatocytes were isolated using collagenase perfusion and then cell viability, mitchondrial membrane potential (MMP) and ROS formation were determined using trypan blue exclusion, Rhodamine 123 fluorescence and fluorogenic probe, 2', 7' -dichlorofluorescin diacetate (DCFH-DA), respectively. RESULTS: Despite the protective effect of NAC on malathion-induced cell toxicity and MMP dysfunction, its efficacy against ROS formation was not adequate to completely protect the cells. CONCLUSION: Cytotoxic effects of malathion regardless of its cholinergic feature, is started with gradual free radical production but, the main factor that causes cell death, is mitochondrial dysfunction, so that reduction of ROS formation alone is not sufficient for cell survival, and the maintenance of mitochondrial integrity through different mechanisms is the most ameliorative factor specially at high levels of cell damage, as NAC seemed to protect cells with various fashions apart from ROS scavenging in concentrations higher than malathion's LC50.

نوع سند :مقاله
زبان سند : انگلیسی
نویسنده اول :سارا مصطفی لو
نویسنده مسئول :محمد علی اقبال
ضریب تاثیر و نمایه مجلات:Indexing in: PubMed , Scopus , Index Copernicus , Ulrichs Web , embase ,Chemical Abstracts Google scholar , Ebsco
کلیدواژه ها (انگلیسی):Organophosphate Pesticides, Cytotoxicity, Oxidative Stress, Mitochondria, N-actylcycteine, Hepatocytes
موضوعات :QV فارماکولوژی > QV 600 سم شناسی
QV فارماکولوژی
بخش های دانشگاهی :دانشکده داروسازی > بخش سم شناسی
کد شناسایی :6266
ارائه شده توسط : دکتر سارا مصطفی لو
ارائه شده در تاریخ :02 بهمن 1393 05:14
آخرین تغییر :02 بهمن 1393 05:14

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