تاثیر ارتباط وارسیانو های MTHFR C677T و eNOS با لیپید پر اکسیداز با پره اکلامپسی

رحیمی, زهره and ملک خسروی, شهره and رحیمی, زیبا and جلیل وند, فرانک and پارسیان, عباس (1391) تاثیر ارتباط وارسیانو های MTHFR C677T و eNOS با لیپید پر اکسیداز با پره اکلامپسی. Clinical Biochemistry ــ 46 (1-2). pp. 143-147. شاپا 0009-9120

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MTHFR C677T and eNOS G894T variants in preeclamptic women: Contribution to lipid peroxidation and oxidative stress

English Abstract

Objectives We aimed to investigate the association between methylenetetrahydrofolate reductase (MTHFR) C677T and endothelial nitric oxide synthase (eNOS) G894T polymorphisms with lipid peroxidation, total antioxidant capacity (TAC) and the risk of preeclampsia in preeclamptic women. Design and Methods We screened a sample of 198 unrelated women with mild and severe forms of preeclampsia and 101 unrelated women with normal pregnancy with the eNOS and MTHFR variants using PCR-RFLP method. Also, the serum malondialdehyde (MDA) and TAC levels were determined using HPLC and commercial kits, respectively. Results The frequency of combined genotypes of MTHFR CT and TT (CT + TT) and T allele tended to be higher in severe preeclamptic women compared to controls. There was no significant difference for eNOS G894T genotype and allele frequencies between patients and controls. A significantly higher level of triglycerides was observed in the presence of combined genotypes of MTHFR CT and TT and also eNOS GT and TT (GT + TT) in preeclamptic women compared to controls with the same genotype. Also, the presence of MTHFR TT genotype in severe preeclamptic women was significantly associated with the increased serum MDA level compared to CC genotype. In severe preeclamptic women the presence of CT and combined genotypes of CT and TT was significantly associated with the decreased TAC level compared to CC genotype. Also, a higher MDA level was observed in mild preeclamptic women with eNOS TT genotype compared to those with GG genotype but the difference was not significant. Conclusion The present study indicates that MTHFR C677T polymorphism through affecting on TG level, lipid peroxidation and oxidative stress might be involved in the pathogenesis of severe preeclampsia.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :زهره رحیمی
نویسنده :فرانک جلیل وند
Additional Information:Impact Factor (2013) 2.229 Indexed in: ISI, Pubmed/Medline/Index Medicus, Scopus, Embase, Chemical Abstracts, Current Contents/Life Sciences, BIOSIS
کلیدواژه ها (انگلیسی):Preeclampsia, MTHFR C677T, eNOS G894T, Lipid peroxidation, TAC
Subjects:WQ Obstetrics
Divisions:Faculty of Medicine > Department of Anesthesiology , Obstetrics & Gynecology
ID Code:10000
Deposited By: دکتر فرانک جلیل وند
Deposited On:29 Feb 1398 16:23
Last Modified:04 Mar 1398 23:49

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