شيوع ژن ermB در بين ایزوله های بالينی استافيلوکوک اورئوس در شهرکرد، ايران

نادری, کوروش and قنبری, فهیمه and خادمی, فرزاد and زمان زاد, بهنام (1396) شيوع ژن ermB در بين ایزوله های بالينی استافيلوکوک اورئوس در شهرکرد، ايران. Journal of Kerman University of Medical Sciences ــ 25 (1). pp. 67-76. شاپا 1023-9510


Official URL: http://jkmu.kmu.ac.ir/article_63178.html


Prevalence of erm Gene among Clinical Isolates of Staphylococcus aureus in Shahrekord, Iran

English Abstract

Background: Development of drug resistance to Staphylococcus aureus (S. aureus) has led to the use of older antibiotics such as macrolide-lincosamide-streptogramin B (MLSB) for the treatment of infections. MLSB resistance can be caused by several mechanisms, however, one of the predominant reasons is target modification mediated by erm genes. The objective of this study is to determine the prevalence of erm genes and the frequency of constitutive MLSB (cMLSB), inducible MLSB (iMLSB), and MS phenotypes using D-test and polymerase chain reaction (PCR) methods. Methods:D-test was performed on 110 clinical specimens of S. aureus collected from Kashani and Hajar Hospitals in Shahrkord from October 2014 to May 2015. After sampling, DNA extraction was performed by simple boiling method and, in order to detect erm genes, multiplex PCR was carried out on erythromycin resistant isolates using specific primers. Results:The result of this study revealed that among 110 S. aureus isolates examined, 35 (31.8%) were MRSA and frequency of cMLSB, iMLSB, and MS resistant phenotypes were 22 (20%), 9 (8.2%), and 2 (1.8%), respectively. The genes ermA, ermB, and ermC were detected in 27 (24.5%), 28 (25.4%), and 26 (23.6%) isolates. Conclusion:This study demonstrated that cMLSB was the most common phenotype among isolated S. aureus. Moreover, another interesting point to notice in our study was the high frequency of the ermB gene in iMLSB resistant phenotypes.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :کوروش نادری
نویسنده :فهیمه قنبری
نویسنده :فرزاد خادمی
نویسنده مسئول :بهنام زمان زاد
Additional Information:Indexed in: Scopus, ISC, Magiran, SID
کلیدواژه ها (انگلیسی):Staphylococcus aureus; D-test; Erm gene; Macrolide-lincosamid-streptogramin
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:10294
Deposited By: Dr Farzad Khademi
Deposited On:07 May 1397 10:06
Last Modified:26 Oct 1401 10:09

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