title

ارزیابی بیان ژن EGFR در جنین های دو سلولی و بلاستوسیست موش آزمایشگاهی بدنبال انجماد سریع یا ویتریفیکیشن

گازر, روح اله and اسکندری, مژگان and شرف شاه, علیرضا and بهادری, محمد هادی and گل محمدی, محمد قاسم and کشاورز, پروانه (1397) ارزیابی بیان ژن EGFR در جنین های دو سلولی و بلاستوسیست موش آزمایشگاهی بدنبال انجماد سریع یا ویتریفیکیشن. Avicenna Journal of Medical Biotechnology ــ 10 (2). pp. 120-122. شاپا 2008-2835

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Title

Assessment of EGFR Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos

English Abstract

Background: Exact mechanisms of fetal harm following vitrification are still unknown. This study was conducted to evaluate the cryopreservation impact on the expression of Epidermal Growth Factor Receptor (EGFR) gene in mouse 2-cell and blastocysts. Methods: To stimulate ovulation in mice, hCG was injected, followed by collecting 2-cells and blastocysts after 44-46 and 88-89 hr, respectively. These embryos were divided into two case and control groups. The fresh case group was cryopreserved using cryotop and warmed after 4 mounts. Normal 2-cells were selected based on their morphology and their RNA was extracted. Quantitative expression of EGFR gene in both groups was investigated by applying real time-PCR. Results: The statistical real-time (RT)-PCR analyses performed using SPSS revealed that the expression level of EGFR gene was diminished in the case group compared to the control group. Conclusion: The current study indicated the negative effect of cryopreservation on expression amount of EGFR gene in 2-cell and blastocyst mouse embryos.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :روح اله گازر
نویسنده :مژگان اسکندری
نویسنده :علیرضا شرف شاه
نویسنده :محمد هادی بهادری
نویسنده :محمد قاسم گل محمدی
نویسنده مسئول :پروانه کشاورز
Additional Information:Indexed in: Pubmed/PMC, Scopus, Scopus, Biological Abstracts, BIOSIS Previews, EMBASE, Index Copernicus, DOAJ, Google Scholar, SID, EMRmedex, IranMedex, Magiran
کلیدواژه ها (انگلیسی):Blastocyst , Cryopreservation , Embryo , Gene expression , Vitrification
Subjects:QS Human Anatomy
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:10315
Deposited By: Dr Mohammad Gasem Golmohammani
Deposited On:07 May 1397 17:10
Last Modified:07 May 1397 17:10

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