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بیان با واسطه آدنوویروسی پروتئین HO-1 در سلولهای بنیادی مزانشیمی سیتوتوکسی سیته را کاهش می دهد و آپوپتوز ناشی از استرس اکسیداتیو را مهار می نماید.

حامدی اصل, پژمان and حلبیان, راحله and بهمنی, پریسا and محمدی پور, مهشید and محمدزاده وردین, محمد and محمدی روشنده, آمنه and جهانیان نجف آبادی, علی and Kuwahara, Yoshikazu and حبیبی رودکنار, مهریار (1391) بیان با واسطه آدنوویروسی پروتئین HO-1 در سلولهای بنیادی مزانشیمی سیتوتوکسی سیته را کاهش می دهد و آپوپتوز ناشی از استرس اکسیداتیو را مهار می نماید. Cell Stress and Chaperones ــ 17 (2). pp. 181-190. شاپا 1355-8145

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Title

Adenovirus-mediated expression of the HO-1 protein within MSCs decreased cytotoxicity and inhibited apoptosis induced by oxidative stresses

English Abstract

The capacity of mesenchymal stem cells (MSCs) to survive and engraft in the target tissue may lead to promising therapeutic effects. However, the fact that the majority of MSCs die during the first few days following transplantation complicates cell therapy. Hence, it is necessary to strengthen the stem cells to withstand the rigors of the microenvironment to improve the efficacy of cell therapy. In this study, we manipulated MSCs to express a cytoprotective factor, heme oxygenase-1 (HO-1), to address this issue. Full-length cDNA of human HO-1 was isolated and cloned into TOPO vector by TOPO cloning reaction. Then, the construct was ligated to gateway adapted adenovirus expression vector by LR recombination reaction. Afterwards, the recombinant virus expressing HO- 1 was produced in appropriate mammalian cell line and used to infect MSCs. The HO-1 engineered MSCs were exposed to hypoxic and oxidative stress conditions followed by evaluation of the cells' viability and apoptosis. Transient expression of HO-1 was detected within MSCs. It was observed that HO-1 expression could protect MSCs against cell death and the apoptosis triggered by hypoxic and oxidative stress conditions. The MSCs-HO-1 retained their ability to differentiate into adipogenic, chondrogenic, or osteogenic lineages. These findings could be applied as a strategy for prevention of graft cell death in MSCs-based cell therapy and is a good demonstration of how an understanding of cellular stress responses can be used for practical applications.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :پژمان حامدی اصل
نویسنده :راحله حلبیان
نویسنده :پریسا بهمنی
نویسنده :مهشید محمدی پور
نویسنده :محمد محمدزاده وردین
نویسنده :آمنه محمدی روشنده
نویسنده :علی جهانیان نجف آبادی
نویسنده :Yoshikazu Kuwahara
نویسنده مسئول :مهریار حبیبی رودکنار
Additional Information:IF: 2.571 Indexing in: ISI, Scopus, PubMed, Embase
کلیدواژه ها (انگلیسی):Mesenchymal stem cells, HO-1, Adenovirus, Oxidative stress, Apoptosis
Subjects:QU Biochemistry
QV pharmacology
WH Hemic and Lymphatic System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:10359
Deposited By: Dr Mohammad Mohammadzadeh
Deposited On:14 Jul 1397 12:36
Last Modified:18 Sep 1399 12:14

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  • بیان با واسطه آدنوویروسی پروتئین HO-1 در سلولهای بنیادی مزانشیمی سیتوتوکسی سیته را کاهش می دهد و آپوپتوز ناشی از استرس اکسیداتیو را مهار می نماید. (deposited 14 Jul 1397 12:36) [Currently Displayed]

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