title

بررسی جهش های kIT و FLT3 در زیرنوع های لوسمی های میلوئیدی حاد

ذاکر, فرهاد and محمدزاده وردین, محمد and محمدی, محمد (1389) بررسی جهش های kIT و FLT3 در زیرنوع های لوسمی های میلوئیدی حاد. Archives of Iranian Medicine ــ 13 (1). pp. 21-25. شاپا 1029-2977

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Title

Detection of KIT and FLT3 mutations in acute myeloid leukemia with different subtypes

English Abstract

Background: Mutations in KIT and fms-like tyrosine kinase 3 genes lead to uncontrolled proliferation of leukemic cells with a poor prognosis. Since, data concerning the incidence and associations with patients characteristics vary amongst different studies, the aim of the present study is to identify and quantify the frequency of mutations in Iranian patients suffering from acute myeloid leukemia. Methods: Internal tandem duplication and D835 mutations in the fms-like tyrosine kinase 3 gene of acute myeloid leukemia patients were studied through polymerase chain reaction and polymerase chain reaction-RFLP analysis. Amplified products for a point mutation in D816 for KIT have also been identified through the polymerase chain reaction-RFLP technique. The mutations in exon 8 of KIT were detected by using the PCR and the Conformational Sensitive Gel Electrophoresis techniques, and amplified products have been confirmed by sequencing techniques. Results: Internal tandem duplication and D835 mutations in the fms-like tyrosine kinase 3 gene occurred in 18 and 6 of AML patients, respectively. Frequencies of mutation were 1.4 and 4.7 in exon 8 and D816 of the KIT gene in acute myeloid leukemia patients. These results were substantially different for various subclasses of French-American-British classification. Conclusion: This study revealed that approximately 30 of acute myeloid leukemia patients have either KIT or fms-like tyrosine kinase 3 genetic mutations. The presence of fms-like tyrosine kinase 3 was significantly associated with M3 morphology and mutations of KIT were significantly associated with M2 and M4 subtypes.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :فرهاد ذاکر
نویسنده :محمد محمدزاده وردین
نویسنده :محمد محمدی
Additional Information:IF(2010):1.348 Indexing in: ISI, Scopus, Pubmed/Medline, Embase
کلیدواژه ها (انگلیسی):Acute Myeloid Leukemia, CSGE, mutations, PCR
Subjects:WH Hemic and Lymphatic System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:10364
Deposited By: Dr Mohammad Mohammadzadeh
Deposited On:25 Apr 1398 11:26
Last Modified:18 Sep 1399 12:20

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