تاثیر میکرووزیکول‌های مشتق از سلول‌های مزانشیمی بنیادی روی افزایش لیز تومور و نیز تحریک سلول‌کشی لنفوسیت T توسط سلول‌های دندریتیک توسط این میکرووزیکول‌ها

رحمانی کوکیا, نسیم and علی پناه مقدم, رضا and دلیرژ, نوروز and مأذنی, محمد (1397) تاثیر میکرووزیکول‌های مشتق از سلول‌های مزانشیمی بنیادی روی افزایش لیز تومور و نیز تحریک سلول‌کشی لنفوسیت T توسط سلول‌های دندریتیک توسط این میکرووزیکول‌ها. Asian Pacific Journal of Cancer Prevention ــ 19 (7). pp. 1895-1902. شاپا 1513-7368


Official URL: http://journal.waocp.org/article_65367.html


Mesenchymal Stromal Stem Cell-Derived Microvesicles Enhance Tumor Lysate Pulsed Dendritic Cell Stimulated Autologous T lymphocyte Cytotoxicity

English Abstract

Background: Immunotherapy is one promising therapeutic strategy against glioma, an aggressive form of brain cancer. Previous studies have demonstrated that multiple tumor antigens exist and can be used to induce tumor specific T cell responses. Furthermore, recently it was shown that TLR4-primed mesenchymal stem cells (MSCs), also known as MSC1, mostly elaborate pro-inflammatory mediators. Compared to MSCs, MSC-derived microvesicles (MVs) have advantageous properties that present them as stable, long lasting effectors with no risk of immune rejection. Therefore, peripheral blood monocyte derived dendritic cells (MoDCs) have been used to load tumor antigens and stimulate T cell mediated responses in the presence of MSC1-derived MVs in vitro. Methods: The B92 tumor cell line was heated to 43°C for 90 min prior to preparation of tumor cell lysates. MVs were purified by differential ultracentrifugation after isolation, stimulation of proliferation and treatment of MSCs. Autologous T cells isolated from non-adherent cells were harvested during the procedure to generate MoDCs and then incubated with heat stressed tumor cell lysate pulsed DCs in the presence of MSC1-derived MVs. T cells were then co-cultured with tumor cells in 96-well plates at a final volume of 200 μl CM at an effector: target ratio of 100:1 to determine their specific cytotoxic activity. Results: Flow cytometric analysis, T cell mediated cytotoxicity showed that heat stressed tumor antigen pulsed MoDCs and MSC1-derived MVs primed T cells elicited non-significantly enhanced cytotoxic activity toward B92 tumor cells (P≥0.05). Conclusion: These findings may offer new insights into tumor antigen presenting technology involving dendritic cells and MSC1-derived MVs. Further exploration of the potential of such nanoscale particles in immunotherapy and in novel cancer vaccine settings appears warranted.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :نسیم رحمانی کوکیا
نویسنده مسئول :رضا علی پناه مقدم
نویسنده مسئول :نوروز دلیرژ
نویسنده :محمد مأذنی
Additional Information:Indexed in: Pubmed/PMC/Medline/Index Medicus, Scopus, Index Copernicus, EMBASE , DOAJ
کلیدواژه ها (انگلیسی):Glial cells; Tumor cell lysate; Dendritic Cells; MSC1-derived MVs; cancer immunotherapy
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
ID Code:10370
Deposited By: Dr Reza Alipanah-Moghadam
Deposited On:15 May 1397 10:06
Last Modified:15 May 1397 10:06

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