تغییرات ساختاری و توده ای شدن آنزیم آمین اکسید از حاوی مس در حضور تری فلورو اتانل

امانی, مجتبی and یوسفی, رضا and موسوی موحدی, علی اکبر and پینتوس, فرانسیسکو and مورا, آنا and فلوریس, جیووانی and کورگانف, بوریس ایوان and صبوری, علی اکبر (1387) تغییرات ساختاری و توده ای شدن آنزیم آمین اکسید از حاوی مس در حضور تری فلورو اتانل. Protein and Peptide Letters ــ 15 (5). pp. 521-527. شاپا 0929-8665

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Structural changes and aggregation process of Cu/containing amine oxidase in the presence of 2,2,2â ²-trifluoroethanol

English Abstract

Conformational and structural changes of lentil seedlings amine oxidase (LSAO) were studied in the presence of trifluoroethanol (TFE) by spectroscopic and analytical techniques. At TFE concentrations up to 5%, the induction of a structural transition from β-sheet to α-helix and up to 10% TFE a structural transition from α-helix to β-sheet as well as inactivation of the enzyme are observed. At TFE concentrations between 10-35%, LSAO proves to be prone to aggregation and beyond 35% TFE leads to a non-native protein structure with a high α-helix content. The obtained results revealed that the aggregation of LSAO is strongly linked to the nature of secondary structures. © 2008 Bentham Science Publishers Ltd.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مجتبی امانی
نویسنده مسئول :علی اکبر موسوی موحدی
Additional Information:cited By (since 1996) 0 Impact Factor= 1.281 Abstracted/Indexed in:ISI,Medline/Pubmde, 2-Scopus,BIOSIS, BIOSIS Previews, Biochemistry & Biophysics Citation Index®, Chemical Abstracts,EMBASE/ Excerpta Medica 3-Biomed Link, Biophysics Citation Index®, Journal Citation Reports/ Science Edition, Science Citation Index, BIOBASE, Current Awareness in Biological Sciences (CABS), Biological Abstracts, ISI Biochemistry, EMBiology, Genamics JournalSeek, Google, Google Scholar, MediaFinder®-Standard Periodical Directory.
کلیدواژه ها (انگلیسی):Amine Oxidase ; TFE ; secondary structure ; Circular Dichroism ; fluorescence ; Aggregation
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:1062
Deposited By: MS Soghra Golmaghani
Deposited On:13 Dec 1388 05:47
Last Modified:30 Jan 1393 12:58

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