مطالعه هیدروفوبیسیته سطحی و سینتیک تودهای شدن کال پروتکتین انسانی در حضور کلسیم

یوسفی, رضا and کبودانیان اردستانی, سوسن and صبوری, علی اکبر and کریمی نیا, آمینا and زینالی, مجید and امانی, مجتبی (1384) مطالعه هیدروفوبیسیته سطحی و سینتیک تودهای شدن کال پروتکتین انسانی در حضور کلسیم. Journal of Biochemistry and Molecular Biology ــ 38 (4). pp. 407-413. شاپا 1225-8687

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Investigation on the surface hydrophobicity and aggregation kinetics of human calprotectin in the presence of calcium

English Abstract

Calcium and zinc binding protein, calprotectin is a multifunctional protein with broad spectrum antimicrobial and antitumoural activity. It was purified from human neutrophil, using a two-step ion exchange chromatography. Since surface hydrophobicity of calprotectin may be important in membrane anchoring, membrane penetration, subunits oligomerization and some biological roles of protein, in this study attempted to explore the effect of calcium in physiological range on the calprotectin lipophilicity. Incubation of human calprotectin (50 μg/ml) with different calcium concentrations showed that 1-anilino-8-naphthalene sulfonic acid (ANS) fluorescence intensity of the protein significantly elevates with calcium in a dose dependent manner, suggesting an increase in calprotectin surface hydrophobicity upon calcium binding. Our study also indicates that calcium at higher concentrations (6, 8 and 10 mM) induces aggregation of human calprotectin. Our finding demonstrates that the starting time and the rate constant of calprotectin aggregation depend on the calcium concentration.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :رضا یوسفی
نویسنده مسئول :سوسن کبودانیان اردستانی
نویسنده :مجتبی امانی
Additional Information:0219-1024 (Electronic)
کلیدواژه ها (انگلیسی):Aggregation, ANS-binding fluorescence, Calcium, Calprotectin, Hydrophobicity, Neutrophil
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:1068
Deposited By: MS Soghra Golmaghani
Deposited On:13 Dec 1388 07:20
Last Modified:20 Jan 1393 13:16

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