title

تفکیک حرارتی و قفلهای کنفورماسیونی سوپراکسیداز دیسموتاز

هونگ, جون and موسوی موحدی, علی اکبر and قورچیان, هدایت اله and امانی, مجتبی and امانلو, مسعود and چیلاکا, فردیناندو چیمکا (1384) تفکیک حرارتی و قفلهای کنفورماسیونی سوپراکسیداز دیسموتاز. Journal of Biochemistry and Molecular Biology ــ 38 (5). pp. 533-538. شاپا 1225-8687

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Title

Thermal dissociation and conformational lock of superoxide dismutase

English Abstract

The kinetics of thermal dissociation of Superoxide dismutase (SOD) was studied in 0.05 M Tris-HCl buffer at pH 7.4 containing 10<sup>-4</sup> EDTA. The number of conformational locks and contact areas and amino acid residues of dimers of SOD were obtained by kinetic analysis and biochemical calculation. The cleavage bonds between dimers of SOD during thermal dissociation and type of interactions between specific amino acid residues were also simulated. Two identical contact areas between two subunits were identified. Cleavage of these contact areas resulted in dissociation of the subunits, with destruction of the active centers, and thus, lost of activity. It is suggested that the contact areas interact with active centers by conformational changes involving secondary structural elements.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :جون هونگ
نویسنده مسئول :علی اکبر موسوی موحدی
Additional Information:cited By (since 1996) 1
کلیدواژه ها (انگلیسی):protein subunit; superoxide dismutase, animal; article; binding site; cattle; chemical structure; chemistry; dimerization; enzyme stability; metabolism; protein conformation; temperature, Animals; Binding Sites; Cattle; Dimerization; Enzyme Stability; Models, Molecular; Protein Conformation; Protein Subunits; Superoxide Dismutase; Temperature, Animalia; Bos taurus
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:1069
Deposited By: MS Soghra Golmaghani
Deposited On:13 Dec 1388 07:28
Last Modified:20 Jan 1393 13:17

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