بررسی ارتباط بین پلی مورفیسم های ناحیه کد شونده ژن TGF-B و سقط مکرر خود به خود

امانی, داور and صمصامی دهقان, عالم تاج and ذوالقدری, ژاله and روانگرد, فاطمه and نیکاوا, نوریو and یوشییرو, کو- ایچیرو and قادری, عباس (1384) بررسی ارتباط بین پلی مورفیسم های ناحیه کد شونده ژن TGF-B و سقط مکرر خود به خود. Journal of Reproductive Immunology ــ 68 (1-2). pp. 91-103. شاپا 0165-0378

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Lack of association between the TGF-β1 gene polymorphisms and recurrent spontaneous abortion

English Abstract

Transforming growth factor-β1 (TGF-β1) is produced by T regulatory lymphocytes (Treg), which play an important role in the physiology of pregnancy. Several polymorphisms of the TGF-β1 gene (TGFB1) have been reported, some with an important correlation with TGF-β1 production and disease severity. We performed an association study between TGFB1 polymorphisms and recurrent spontaneous abortion (RSA). We first used a PCR-RFLP method to detect three known TGFB1 cSNPs (coding single nucleotide polymorphisms) among 111 RSA and 110 normal control women from Southern Iran, such as 29T � C (Leu 10 Pro), 74G � C (Arg 25 Pro) and 788 C � T (Thr 263Ile), and compared their frequencies between the two groups of subjects. To confirm results of the RFLP study and to identify new SNPs in the RSA women, we then sequenced their DNA samples for seven exons and adjacent intronic regions of TGFB1. Consequently, 10 SNPs were detected; one (-14G � A) was located in the upstream region of exon 1, three in exons (two in exon 1 and one in exon 5) and six in intronic regions. Two (IVS5 + 18G � C and IVS6 + 910G � A) of the 10 SNPs were novel. Statistical analysis on the frequency of six most frequent SNPs, including the three cSNPs, as well as on the frequencies of genotypes and 13 haplotypes regarding the 6 SNPs, revealed no significant difference between RSA and control women. Therefore, this study concludes that there is no association between exonic and adjacent intronic polymorphisms of TGFB1 and RSA. © 2005 Elsevier Ireland Ltd. All rights reserved.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :داور امانی
Additional Information:cited By (since 1996) 4
کلیدواژه ها (انگلیسی):transforming growth factor beta1, adolescent; adult; article; comparative study; controlled study; correlation analysis; disease severity; exon; female; genetic polymorphism; genotype; haplotype; human; intron; lymphocyte function; physiology; polymerase chain reaction; pregnancy; priority journal; restriction fragment length polymorphism; single nucleotide polymorphism; spontaneous abortion; statistical analysis; T lymphocyte, Abortion, Habitual; Adolescent; Adult; Aged; Exons; Female; Humans; Introns; Middle Aged; Polymorphism, Single Nucleotide; Predictive Value of Tests; Pregnancy; T-Lymphocytes, Regulatory; Transforming Growth Factor beta; Transforming Growth Factor beta1
Subjects:WP Gynecology
WQ Obstetrics
QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Immunology
ID Code:1077
Deposited By: Dr Davar Amani
Deposited On:14 Dec 1388 09:30
Last Modified:07 Jul 1393 05:05

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