میر-124 با سرکوب بیان ژن های Ptbp1 و Sox9 باعث تمایز نورونی در سلولهای بنیادی بالج می شود

مکبر, هاله and نجف زاده, نوروز and محمدزاده وردین, محمد (1397) میر-124 با سرکوب بیان ژن های Ptbp1 و Sox9 باعث تمایز نورونی در سلولهای بنیادی بالج می شود. Journal of Cellular Physiology ــ 234 (6). pp. 8941-8950. شاپا 0021-9541

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Official URL: https://onlinelibrary.wiley.com/doi/pdf/10.1002/jc...


miR‐124 promotes neural differentiation in mouse bulge stem cells by repressing Ptbp1 and Sox9

English Abstract

Hair follicle stem cells (HFSCs) are able to differentiate into neurons and glial cells. Distinct microRNAs (miRNAs) regulate the proliferation and differentiation of HFSCs. However, the exact role of miR‐124 in the neural differentiation of HFSCs has not been elucidated. HFSCs were isolated from mouse whisker follicles. miR‐9, let‐7b, and miR‐124, Ptbp1 , and Sox9 expression levels were detected by real‐time polymerase chain reaction (RT‐PCR). The influence of miR‐124 transfection was evaluated using immunostaining. We demonstrated that miR‐124 and let‐7b expression levels were significantly increased after the neural differentiation. Sox9 and Ptbp1 were identified as the target of miR‐124 in the HFSCs. During neural differentiation and miR‐124 mimicking, Ptbp1 and Sox9 levels were decreased. Moreover, the miR‐124 overexpression increased MAP2 (58.43 ± 11.26) and NeuN (48.34 ± 11.15) proteins expression. The results demonstrated that miR‐124 may promote the differentiation of HFSCs into neuronal cells by targeting Sox9 and Ptbp1.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :هاله مکبر
نویسنده مسئول :نوروز نجف زاده
نویسنده :محمد محمدزاده وردین
Additional Information:Impact Factor (2017)3.923 Indexed in: ISI, Pubmed/Medline/Index Medicus, Scopus,Embase,
کلیدواژه ها (انگلیسی):Sox9, PtbP1, HFSCs, miR-124, neural differentiation
Subjects:QS Human Anatomy
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:10792
Deposited By: Dr Nowruz Najafzadeh
Deposited On:23 Aug 1397 10:18
Last Modified:18 Sep 1399 11:19

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