title

واسرشتگی برگشت ناپذیر کربونیک انهیدراز II انسانی

امانی, مجتبی and خدارحمی, رضا and محرابی, معصومه and قبادی, سیروس and موسوی موحدی, علی اکبر (1388) واسرشتگی برگشت ناپذیر کربونیک انهیدراز II انسانی. در: دهمین کنگره بیوشیمی و سومین کنگره بین المللی بیوشیمی و ملکولی, پاییز 88, تهران.

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Title

Irreversible thermal denaturation of human carbonic anhydrase

English Abstract

Carbonic Anhydrase (CA; carbonate hydro-lyase, EC 4.2.1.1)), a zinc containing enzyme, catalyzes reversible hydration of carbon dioxide. This metalloenzyme is found in animals, photosynthesizing organisms, and in some nonphotosynthetic bacteria. Thermal denaturation of the protein was studied by enzymatic activity, circular dichroism, and differential scanning calorimetry. The results showed that CA thermal denaturation is an irreversible process. Fitting DSC data and consideration of the validity of two-state model realized to conclude CA undergoes irreversible thermal denaturation in one step. Using this model activation energy, T* parameter (the absolute temperature at which the rate constant of denaturation is equal to 1 min−1), and total enthalpy of CA denaturation were calculated.

Item Type:Conference or Workshop Item (Speech)
زبان سند : انگلیسی
نویسنده مسئول :مجتبی امانی
کلیدواژه ها (انگلیسی):carbonic anhydrase, DSC, thermal denaturation, irreversibility
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:1103
Deposited By: Dr Mojtaba Amani
Deposited On:19 Dec 1388 07:09
Last Modified:21 Jan 1393 09:42

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