title

فرمولاسیون و بهینه سازی یک نانوذره جدید PLGA تغییر یافته با لیپید کاتیونیک به عنوان سیستم دلیوری پروتئین فیوژن HspX/EsxS مایکوباکتریوم توبرکلوزیس: یک طراحی آزمایشگاهی

خادمی, فرزاد and یوسفی اوروند, ارشید and درخشان, محمد and عباسپور, محمدرضا and صدری, کیوان and تفقدی, محسن (1397) فرمولاسیون و بهینه سازی یک نانوذره جدید PLGA تغییر یافته با لیپید کاتیونیک به عنوان سیستم دلیوری پروتئین فیوژن HspX/EsxS مایکوباکتریوم توبرکلوزیس: یک طراحی آزمایشگاهی. Iranian Journal of Pharmaceutical Research ــ 18 (1). pp. 446-458. شاپا 1735-0328

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Title

Formulation and optimization of a new cationic lipid-modified PLGA nanoparticle as delivery system for Mycobacterium tuberculosis HspX/EsxS fusion protein: An experimental design

English Abstract

Polymeric particles and liposomes are efficient tools to overcome the low immunogenicity of subunit vaccines. The aim of the present study was formulation and optimization of a new cationic lipid-modified PLGA nanoparticles (NPs) as a delivery system for Mycobacterium tuberculosis HspX/EsxS fusion protein. The cationic lipid-modified PLGA NPs containing HspX/EsxS fusion protein were prepared using a modified double emulsion solvent evaporation method. Scanning electron microscopy and dynamic light scattering (DLS) tools were used to determine physical properties of hybrid NPs. A multi-level full factorial design was used to evaluate the influence of two factors of PLGA:DDA ratio (w/w) and PVA concentration (%) on size, surface charge, polydispersity index, encapsulation efficiency and yield. Finally, the optimal formulation was achieved based on desired responses. Mathematical models were obtained to indicate the relation between the studied factors and responses. The DDA concentration showed an increasing effect on surface charge and also a decreasing effect on particle size, encapsulation efficiency and yield. Higher amounts of DDA, increased surface charge of NPs, however, the size, encapsulation efficiency and yield were decreased. The influence of various concentrations of PVA on different physical characteristics of PLGA:DDA hybrid NPs was variable. The optimal formulation was consisted of 0.91 (55:5, w/w) ratio of PLGA:DDA and 0.5% PVA. The hybrid NPs showed acceptable particle size distribution, strong positive surface charge, prolonged antigen release and good encapsulation efficiency in comparison to PLGA alone. However, further preclinical and clinical studies are needed.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :فرزاد خادمی
نویسنده :ارشید یوسفی اوروند
نویسنده :محمد درخشان
نویسنده :محمدرضا عباسپور
نویسنده :کیوان صدری
نویسنده مسئول :محسن تفقدی
Additional Information:Impact Factor (2017) 1.372 Indexed in: ISI, Pubmed/PMC, Scopus, Embase, Chemical Abstracts, Google Scholar, Index Copernicus, IranMedex, ISC, Magiran, SID
کلیدواژه ها (انگلیسی):Mycobacterium tuberculosis; PLGA:DDA hybrid nanoparticle; HspX/EsxS fusion protein; Experimental design, Optimization
Subjects:QV pharmacology > QV 704 Pharmaceutics
QV pharmacology
QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:11365
Deposited By: دکتر فرزاد خادمی
Deposited On:23 Nov 1397 14:06
Last Modified:23 Nov 1397 14:06

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