فعالیت ضد میکروبی درمان فتودینامیک در ترکیب با کلستین بر علیه Acinetobacter baumannii بسیارمقاوم جدا شده از بیمار سوختگی

بلوکی, ابراهیم and کاظمیان, حسین and پیری دوگاهه, هادی and علیخانی, محمد یوسف and شهابی, سیما and بیت الهی, لیلی and قربان زاده, رقیه (1396) فعالیت ضد میکروبی درمان فتودینامیک در ترکیب با کلستین بر علیه Acinetobacter baumannii بسیارمقاوم جدا شده از بیمار سوختگی. Photodiagnosis and Photodynamic Therapy ــ 18 . pp. 1-5. شاپا 1572-1000

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Antimicrobial activity of photodynamic therapy in combination with colistin against a pan-drug resistant Acinetobacter baumannii isolated from burn patient

English Abstract

Nosocomially-acquired multi-, extensively-, and pandrug resistant (MDR, XDR, and PDR) strains of microorganisms such as Acinetobacter baumannii remain a serious cause of infection and septic mortality in burn patients. Treatment of patients with nosocomial burn wound infections is often complicated by drug-resistant strains of A. baumannii. Today, many researchers are focusing on the investigation of novel non-antibiotic strategies such as photodynamic therapy (PDT). We report a new PDT strategy that suppresses colistin resistance in PDR A. baumannii by interfering with the expression of a pmrA/pmrB two-component system. In the current study, A. baumannii with a PDR feature isolated from a burn patient was used as a test strain. PDT was carried out using toluidine blue O (TBO) and light-emitting diode (LED) as a photosensitizer and radiation source, respectively. The antimicrobial susceptibility profiles were assessed for cells surviving PDT. The effects of sub-lethal PDT (sPDT) on the expression of the pmrA/pmrB two-component signal transduction system were evaluated by real-time quantitative reverse transcription PCR. Results of drug susceptibly testing (DST) in LED and TBO groups separately showed that the bacteria were resistant to all tested antibiotics, while the DST result of the LED + TBO group showed highly declining bacterial growth when compared with the control group. Reduction in the expression of pmrA and pmrB was observed in the treated strains after sPDT. This represents the first conclusive example of a direct role for the PDT in breaking antibiotic resistance by directly modulating two-component system activity.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :ابراهیم بلوکی
نویسنده :هادی پیری دوگاهه
نویسنده مسئول :رقیه قربان زاده
Additional Information:Impact Factor(2017) 2.895 Indexed in: ISI, Pubmed/Medline/Index Medicus, , Embase, Current Contents / Clinical Medicine, ScienceDirect
کلیدواژه ها (انگلیسی):Acinetobacter baumannii, Antimicrobial resistance, Expression, Photodynamic therapy, qRT-PCR, Sub-lethal, pmrA, pmrB
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:11573
Deposited By: Ms Zeynab Imani
Deposited On:21 Dec 1397 14:59
Last Modified:21 Dec 1397 14:59

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