عوامل محافظت کننده میتوکندریایی و آنتی اکسیدان ها از سمیت ناشی از اورانیوم ضعیف شده در لنفوسیت های انسانی محافظت میکنند.

سلطانی, مونا and زارعی, محمد هادی and سلیمی, احمد and پوراحمد, جلال (1398) عوامل محافظت کننده میتوکندریایی و آنتی اکسیدان ها از سمیت ناشی از اورانیوم ضعیف شده در لنفوسیت های انسانی محافظت میکنند. Journal of Environmental Radioactivity ــ 203 . pp. 112-116. شاپا 0265-931X

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Mitochondrial protective and antioxidant agents protect toxicity induced by depleted uranium in isolated human lymphocytes

English Abstract

Depleted uranium (DU) is a by-product of the enrichment procedure of natural uranium. During production and usage, uranium may be released into the environment due to failure to follow standard procedures, thus causing environmental pollution. In this study, toxicity effects of uranium (VI) and protective role of mitochondrial permeability transition pore sealing and antioxidant agents studied by isolated human lymphocytes. Human lymphocytes were exposed to different concentrations (0.1, 0.5, 1, 2 and 5 mM) of DU for 6 h and cytotoxicity was measured by trypan blue assay. The mechanistic parameters were assessed after 1, 2 and 3 h of lymphocyte treatment with 1/2 IC506h (0.3 mM), IC506h (0.8 mM) and 2 IC506h (1.6 mM) of DU. The reactive oxygen species (ROS), lysosomal membrane destabilization, mitochondrial membrane potential (MMP), lipid peroxidation, GSH and GSSG levels on human lymphocytes exposed to UA, were measured. The results indicate that toxicity of U (VI) was concentration dependent on human lymphocytes. Also, U (VI) induced ROS production, MMP reduction, lysosomal membrane destabilization and lipid peroxidation in human lymphocytes. In U (VI) treated lymphocytes, decrease in intracellular GSH and raise in extracellular GSSG levels were observed. We report that mitochondrial permeability transition (MPT) pore sealing and antioxidant agents, have the capacity significantly to prevents, mitochondrial toxicity. Thus, the inhibition of mitochondrial oxidative stress and mitochondrial dysfunction by MPT pore sealing and antioxidant agents is associated with the inhibition of DU-induced mitochondrial damages and activation of apoptosis in lymphocytes.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مونا سلطانی
نویسنده :محمد هادی زارعی
نویسنده مسئول :احمد سلیمی
نویسنده مسئول :جلال پوراحمد
Additional Information:IF(2017): 2.263 Indexing in: ISI, Pubmed/Medline, Scopus, Index medicus, Google scholar
کلیدواژه ها (انگلیسی):Cytotoxicity, Depleted uranium(Uranium VI,Mitochondria, Human lymphocyte
Subjects:QV pharmacology > QV 600 Toxicology
QV pharmacology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:11894
Deposited By: دکتر احمد سلیمی
Deposited On:20 Mar 1398 12:12
Last Modified:20 Mar 1398 12:13

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