سنتز و ارزیابی اثر ضدمیکروبی نانوذرات مغناطیسی تغییر یافته poly-(amidoamine) G5 بر اشریشیا کلی و استافیلوکوکوس اورئوس

نظری, شهرام and غلامی, میترا and فرزادکیا, مهدی and اکبری دورباش, فخرالدین and ارزنلو, محسن and رضایی کلانتری, روشنک (1397) سنتز و ارزیابی اثر ضدمیکروبی نانوذرات مغناطیسی تغییر یافته poly-(amidoamine) G5 بر اشریشیا کلی و استافیلوکوکوس اورئوس. Journal of Molecular Liquids ــ 276 . pp. 93-104. شاپا 0167-7322

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Synthesis and evaluation of the antibacterial effect of silica-coated modified magnetic poly-(amidoamine) G5 nanoparticles on E. coli and S. aureus

English Abstract

Recently, magnetic nanoparticles have been considered to bacterial removal, due to monodisperse size, the ability of functionalization or modification. A novel amino-terminated poly (amidoamine) generation 5 (PAMAMG5) functionalized Fe3O4/SiO2- GPTMS magnetic nanomaterial with a core–shell structure was developed. The nanocomposite was employed to determine antibacterial properties using paper disc diffusion, broth microdilution (MinimumInhibitory Concentration-MIC andMinimumBactericidal Concentration -MBC determination), adsorption, and surface plate count (Colony Forming Unit-CFU/ml) methods. The nanocomposite was characterized by FTIR,N2 adsorption/desorption, Zeta potential, FE-SEM, TEM, EDX,DLS, XRD, TGA and VSMtechniques. The impacts of different parameters including primary bacteria count, solution pH, reaction time and the nanocomposite dosage were studied. The nanocomposite cytotoxicity was investigated on AGS cell line by MTT assay. Nanocomposite was effective in preventing both bacterial strains growth. The MIC and MBC values for E. coli and S. aureus were determined 4 and 8 μg/ml and 16 μg/ml for both strains respectively. Synthesized nanocomposite as antibacterial in an aqueous solution, was elimination effectiveness of 100% for both selected bacteria in optimum condition (pH = 7, nanocomposite dosage = 16 μg/ml, run time = 60 min, and initial bacteria count=103 CFU/ml). FE-SEM analysis revealedmorphological variations and themechanism of killing and trapping the bacteria by nanocomposite. The nanocomposite cytotoxicity on the cell indicated the impact of exposure time and nanocomposite concentration. The reduction in cell viability in the concentration of 16 μg/ml after 48 and 72 h treatment was shown to be 27.4 and 28.7%, respectively. The nanocomposite is able to reduce the E. coli and S. aureus count in drinking water, which meets the drinking water standards according to WHO guidelines.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :شهرام نظری
نویسنده مسئول :میترا غلامی
نویسنده :مهدی فرزادکیا
نویسنده :فخرالدین اکبری دورباش
نویسنده :محسن ارزنلو
نویسنده :روشنک رضایی کلانتری
Additional Information:IF(2018):4.561 Indexing in: ISI, Scopus, Google scholar
کلیدواژه ها (انگلیسی):Magnetic core-shell nanoparticles, Escherichia coli, Staphylococcus aureus, GPTMSAntibacterial
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:11971
Deposited By: Dr Mohsen Arzanlou
Deposited On:09 Apr 1398 09:34
Last Modified:09 Apr 1398 09:34

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