title

2,4-D باعث القا استرس اکسیداتیو و اپوپتور در سلول های بنیادی پالپ دندان می شود

محمودی نیا, سمیرا and نیاپور, علی and قاسمی حمیدآبادی, هاتف and مأذنی, محمد (1398) 2,4-D باعث القا استرس اکسیداتیو و اپوپتور در سلول های بنیادی پالپ دندان می شود. Environmental Science and Pollution Research ــ . شاپا 0944-1344 (In Press)

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Official URL: https://link.springer.com/article/10.1007/s11356-0...


Title

2,4-D causes oxidative stress induction and apoptosis in human dental pulp stem cells (hDPSCs)

English Abstract

2,4-Dicholorophenoxy acetic acid (2,4-D) is a worldwide used hormone herbicide. Human dental pulp stem cells (hDPSCs) as a potential source of mesenchymal stem cells provide a confident model system for the assessments of chemicals in vitro. The main objective of this study was to examine the biological effects and damages attributed to 2,4-D on hDPSCs. hDPSCs were isolated from third molar pulp tissues and their mesenchymal identity were evaluated. Then, hDPSCs were treated with increasing concentrations of 2,4-D (0.1 μM-10 mM). Cell viability assay and cumulative cell counting were carried out to address 2,4-D effects on biological parameters of hDPSCs. Cell cycle distribution, ROS level and ALP activity were measured before and after treatment. AO/EB staining and caspase 3/7 activity were investigated to detect the possible mechanisms of cell death. Flow-cytometric immunophenotyping and differentiation data confirmed the mesenchymal identity of cultivated hDPSCs. 2,4-D treatment caused a hormetic response in the viability and growth rate of hDPSCs. G0/G1 cell cycle arrest, enhanced ROS level, and reduced ALP activity were detected in hDPSCs treated with EC50 dose of 2,4-D. AO/EB staining showed a higher percentage of alive cells in lower concentrations of the herbicide. The increment in 2,4-D dose and the number of early and late apoptotic cells were increased. DAPI staining and caspase 3/7 assay validated the induction of apoptosis. 2,4-D concentrations up to 100 μM did not affect hDPSCs viability and proliferation. The intense cellular oxidative stress and apoptosis were observed at higher concentration.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سمیرا محمودی نیا
نویسنده مسئول :علی نیاپور
نویسنده :هاتف قاسمی حمیدآبادی
نویسنده :محمد مأذنی
Additional Information:IF(2018):2.914 Indexing in: ISI, Pubmed/medline, Scopus
کلیدواژه ها (انگلیسی):Mesenchymal stromal cells, hDPSCs, 2,4-D, Viability, Hormesis, Apoptosis
Subjects:QV pharmacology > QV 600 Toxicology
QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:12041
Deposited By: Dr Ali Niapour
Deposited On:27 Apr 1398 10:29
Last Modified:27 Apr 1398 10:34

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