حاملی مدفوعی گونه های انتروکوک مقاوم به سطوح بالای آمینوگلیکوزیدها و آمپی سیلین در بچه های سالم ایرانی

عنوان انگليسی

Fecal Carriage of High-Level Aminoglycoside and Ampicillin-Resistant Enterococcus Species in healthy Iranian children

خلاصه انگلیسی

Objectives High-level aminoglycoside, ampicillin and vancomycin resistance and virulence genes among enterococci isolates collected from healthy middle-school children in Ardabil, Iran, were investigated. Methods Totally, 305 fecal specimens were collected during 2017. Isolates were subjected to antimicrobial susceptibility testing, detection of virulence genes and molecular typing. Results Totally, 409 isolates were collected with E. faecium as the most common species 235(77%), followed by E. faecalis 56(18%) and other Enterococcus spp. 118(38%). Overall, 71(17.3%), 11(2.7%) and 10(2.4%) of the isolates were identified as high-level streptomycin-resistant (HLSR), high-level gentamicin-resistant (HLGR) and ampicillin-resistant (AR) Enterococcus spp., respectively. From HLSR isolates; 42(59%), 4(5.63%) and 25(35.2%) were E. faecium, E. faecalis and other Enterococcus spp., respectively. Of the HLGR isolates; 4(36.4%) and 7(63.6%) and AR isolates; 7(70%) and 3(30%) were including E. faecium and other Enterococcus spp. respectively. Accordingly, 23.3%, 3.6% and 3% of subjects were found to be colonized with HLSR-, HLGR- and AR-Enterococcus spp. Carriage of HLGR, HLSR and AR isolates were associated with prior antibiotic consumption (P < 0.05). Additionally, male gender and anti-acid consumption were associated with AR-enterococci carriage. Sixty-nine (97.9%) and 9(90%) of the HLSR and HLGR isolates were multidrug-resistant, respectively. No vancomycin-resistant enterococci were detected. ERIC-PCR revealed high genetic diversity among the isolates. gelE and asa1 were the major virulence genes in both E. faecalis and E. faecium isolates. The presence of gelE, was associated with HLSR and HLGR phenotypes (P < 0.05). Conclusions The intestinal carriage of HLSR-enterococci was high in our community. However, carriage with HLGR and AR-enterococci is low.

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