هدف قرار دادن تخریب و دمیلینه شدن آکسون با استفاده از تجویز همزمان 17بتا استرادیول و سلول های شوآن در مدل موشی ضایعه نخاعی

نامجو, زینب and مرتضایی, کیوان and جغتایی, محمد تقی and مرادی, فاطمه and پیریایی, عباس and عباسی, یوسف and حسینی, امیر and مجیدپور, جمال (1397) هدف قرار دادن تخریب و دمیلینه شدن آکسون با استفاده از تجویز همزمان 17بتا استرادیول و سلول های شوآن در مدل موشی ضایعه نخاعی. Journal of Cellular Biochemistry ــ 119 (12). pp. 10195-10203. شاپا 0730-2312

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Targeting axonal degeneration and demyelination using combination administration of 17β-estradiol and Schwann cells in the rat model of spinal cord injury

English Abstract

Schwann cells (SCs) are known to be responsible for axonal ensheathing and myelination, and their transplantation is used commonly for treatment of spinal cord injury (SCI). 17β‐estradiol (E2) has also reported for its protective roles in neurons in the transplanted SCs to the SCI model. In the current study, we evaluated the roles of E2 administration before SCs transplantation in targeting SCI‐induced axonal degeneration and demyelination. E2 (25µg/kg, IP) was administered to the male Wistar rats underwent contusive SCI at T10 segment. At 7 days after injury, 1×106 SCs were transplanted to the injury epicenter of the spinal cord. The groups were laminectomy, SCI, SCI+E2, and SCI+E2+SCs. Functional recovery was evaluated using the Basso‐Bresnahan‐Beattie locomotor test. Sections from spinal cord were also assessed for histoloical staining, including Luxol fast blue, Bielschowskyʼs silver and immunofluorescence evaluation of myelin basic protein (MBP). The SCI group showed impaired locomotion in the hind limb, increased number of cavities within spinal cord, low observable numbers of regenerating fibers, and a significant decrease in the rate of expression for MBP. These changes were counteracted in the treatment groups (P<0.05 vs SCI) with no significant changes among them. From the results, it may be concluded that application of E2 and SCs could be effective when axons undergo demyelination and degenerative processes, and their combination could partly provide cumulative outcomes.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :زینب نامجو
نویسنده :کیوان مرتضایی
نویسنده مسئول :محمد تقی جغتایی
نویسنده :فاطمه مرادی
نویسنده :عباس پیریایی
نویسنده :یوسف عباسی
نویسنده :امیر حسینی
نویسنده :جمال مجیدپور
Additional Information:IF:3.448 Indexed in:ISI, Scopus, PubMed/Medline, Embase
کلیدواژه ها (انگلیسی):17β-estradiol (E2); Schwann cells (SCs); myelin basic protein (MBP); spinal cord injury (SCI)
Subjects:QV pharmacology
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:12157
Deposited On:13 Jun 1398 14:51
Last Modified:16 Jun 1398 08:54

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