واکسن پپتیدی جدید پیشگیری کننده HPV، طراحی شده توسط روش های ایمونوانفورماتیک و واکسینولوژی ساختاری

نگهداری پور, مانیکا and اسلامی, محبوبه and نظافت, نوید and حاجی قهرمانی, نسیم and قشون, محمدباقر and شولیان, اسکندر and دهشهری, علی and عرفانی, نصرالله and مروت, محمد حسین and قاسمی, یونس (1396) واکسن پپتیدی جدید پیشگیری کننده HPV، طراحی شده توسط روش های ایمونوانفورماتیک و واکسینولوژی ساختاری. Infection Genetics and Evolution ــ 54 (2017). pp. 402-416. شاپا 15671348

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A novel HPV prophylactic peptide vaccine, designed by immunoinformatics and structural vaccinology approaches

English Abstract

Human papillomavirus (HPV)-caused cervical cancer is the fourth common female cancer globally. Despite availability of three effective vaccines in market, development of HPV prophylactic vaccines is still pursued due to affordability issues and type-restricted protection of the marketed vaccines. Investigational second generation prophylactic HPV vaccines are mostly exploiting epitopes from the virus minor capsid protein (L2), which despite many advantages suffer from low immunogenicity, a common problem of epitope vaccines. Adjuvants such as TLR agonists may overcome this drawback. In this study, different immunoinformatics and computational tools were employed to design a novel peptide vaccine for protection against cervical cancer. Two immunodominant epitope domains (amino acids 10–36 and 65–89) from the L2 protein of HPV 16 with potential to promote Th1, Th2, CTL, B-cell, and INF-gamma responses were selected. Flagellin, as a TLR5 agonist, a short synthetic TLR4 agonist, and two universal T-helper agonists (PADRE and TpD) were added to ensure strong induction of immune responses. Different segments were joined by proper linkers, and the physicochemical, structural, and immunological characteristics of the resultant construct were evaluated. Modeling, refinement, and validation were done to achieve a high quality 3D structure of the vaccine protein. Docking and molecular dynamics (MD) studies demonstrated an appropriate and stable interaction between the vaccine and TLR5 during the simulation period. Totally, a potential vaccine candidate with proper immunological and physicochemical properties was designed for HPV prophylaxis. The designed vaccine is expected to be capable of generating humoral and cellular responses, which are vital for protection against HPV.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مانیکا نگهداری پور
نویسنده :محبوبه اسلامی
نویسنده :نوید نظافت
نویسنده :نسیم حاجی قهرمانی
نویسنده :محمدباقر قشون
نویسنده :اسکندر شولیان
نویسنده :علی دهشهری
نویسنده :نصرالله عرفانی
نویسنده :محمد حسین مروت
نویسنده مسئول :یونس قاسمی
Additional Information:Impact Factor 2018 : 2.545 Indexed in: ISI, Scopus , Embase , BIOSIS Previews
Uncontrolled Keywords:واکسن پاپیلوما ویروس انسانی، سرطان سرویکس، طراحی واکسن، ایمونوانفورماتیک
کلیدواژه ها (انگلیسی):Human papillomavirus (HPV) vaccine , Minor capsid protein (L2), Cervical cancer (CxCa) , Immunoinformatics , Vaccine design
Subjects:QV pharmacology > QV 737 Pharmaceutical Biotechnology
Divisions:School of Pharmacy > Department of Pharmaceutical Biotechnology
ID Code:12329
Deposited By: دکتر نسیم حاج قهرمانی
Deposited On:04 Sep 1398 16:10
Last Modified:04 Sep 1398 16:10

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