بررسی سیستم های CRISPR-Cas در Clostridium botulinum از طریق ابزارهای بیوانفورماتیک

نگهداری پور, مانیکا and نظافت, نوید and حاجی قهرمانی, نسیم and رحمت آبادی, سید سهیل and قاسمی, یونس (1396) بررسی سیستم های CRISPR-Cas در Clostridium botulinum از طریق ابزارهای بیوانفورماتیک. Infection Genetics and Evolution ــ 54 . pp. 355-373. شاپا 15671348

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Investigating CRISPR-Cas systems in Clostridium botulinum via bioinformatics tools

English Abstract

The Clustered regularly interspaced short palindromic repeats (CRISPR) systems are a type of innate immunity found in some prokaryotes, which protect them against alien genetic elements by targeting foreign nucleic acids. Some other functions are also attributed to these systems. Clostridium botulinum bacteria produce botulinum neurotoxins (BoNT), one of the deadliest known toxins for humans and some animals. Food poisoning due to these bacteria is still a challenge in food industries. On the other hand, BoNT has been widely investigated for therapeutic applications including different muscle disorders. Bont genes may be located on bacterial chromosomes, plasmids, or even prophages. Generally, the genomes of Cl. botulinum show a high level of plasticity. In order to investigate the presence and characteristics of CRISPRs in these anaerobe bacteria, an in silico study on 113 CRISPR arrays identified in 38 Cl. botulinum strains was performed. A high occurrence of CRISPR arrays (80%) were found, with a remarkable frequency on plasmids. Several (CRISPR-associated) Cas proteins from different types were recognized in the studied strains, which were mostly Cas6. The CRISPR-Cas systems were identified as type I or III, but no type II. The spacers showed more homology with bacterial plasmids than phages. Active CRISPR-Cas systems can prevent the transfer of foreign genes, which may also include bont genes. This study provides the first insight into the probable roles of CRISPR-Cas systems in Cl. botulinum strains such as toxigenicity.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مانیکا نگهداری پور
نویسنده :نوید نظافت
نویسنده :نسیم حاجی قهرمانی
نویسنده :سید سهیل رحمت آبادی
نویسنده مسئول :یونس قاسمی
Additional Information:Impact factor 2018: 2.545 Indexed in : ISI , Scopus , Embase , BIOSIS Previews
کلیدواژه ها (انگلیسی):Botulinum toxin , Bioinformatics , Clostridium botulinum CRISPR (clustered regularly interspaced short palindromic repeats) , Cas (CRISPR-associated) proteins , Direct repeat
Subjects:QV pharmacology > QV 737 Pharmaceutical Biotechnology
Divisions:School of Pharmacy > Department of Pharmaceutical Biotechnology
ID Code:12334
Deposited By: Dr Nasim Hajighahramani
Deposited On:04 Sep 1398 16:11
Last Modified:04 Sep 1398 16:11

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