title

مطالعات اینسیلیکو سیگنال پپتیدهای مختلف جهت تولید ترشحی اینترلوکین 11 در باکتری Escherichia coli

نگهداری پور, مانیکا and نظافت, نوید and حاجی قهرمانی, نسیم and رحمت آبادی, سید سهیل and مروت, محمد حسین and قاسمی, یونس (1396) مطالعات اینسیلیکو سیگنال پپتیدهای مختلف جهت تولید ترشحی اینترلوکین 11 در باکتری Escherichia coli. Current Proteomics ــ 14 (1). pp. 1-10. شاپا 15701646

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Title

In Silico Study of Different Signal Peptides for Secretory Production of Interleukin-11 in Escherichia coli

English Abstract

Secretory production of heterologous proteins in bacterial hosts has been a topic of interest due to its various advantages. However, it is difficult to achieve because of process complexity and the need for selection of an appropriate signal peptide for each protein and host. IL- 11, marketed as Neumega®, is a multifunctional cytokine approved for platelet recovery in chemotherapy-induced thrombocytopenia. The in silico evaluation of 30 signal peptides for finding the best theoretical candidates for the secretory production of recombinant IL-11 in Escherichia coli. The prediction of signal peptide presence and location of cleavage sites were done by SignalP 4.1. Five signal peptides (ompT, npr, TorA, caf1M, and phoA) were excluded from further evaluations due to cleavage issues. Different physicochemical properties of signal peptides, which may affect protein secretion, were studied by ProtParam and PROSO II servers. Computational analysis of the influencing factors identified TorT, sfmC, and ompC, respectively, as the best theoretical candidates for the secretory production of IL-11 in E. coli. However, in the experimental investigation, other influencing factors and a system biology approach should be considered.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مانیکا نگهداری پور
نویسنده :نوید نظافت
نویسنده :نسیم حاجی قهرمانی
نویسنده :سید سهیل رحمت آبادی
نویسنده :محمد حسین مروت
نویسنده مسئول :یونس قاسمی
Additional Information:Impact factor: 0.606 , Indexed in : ISI(Science Citation Index Expanded) BIOSIS Previews , Scopus , EMBASE , Chemical Abstracts , ProQuest
Uncontrolled Keywords:اینترلوکین-11، اینسیلیکو، اپرلوکین، پپتید نشانه، تولید ترشحی
کلیدواژه ها (انگلیسی):Interleukin-11, in silico, oprelvekin, secretory production, signal peptide, SRP-pathway
Subjects:QV pharmacology > QV 737 Pharmaceutical Biotechnology
Divisions:School of Pharmacy > Department of Pharmaceutical Biotechnology
ID Code:12348
Deposited By: Dr Nasim Hajighahramani
Deposited On:04 Sep 1398 16:17
Last Modified:20 Nov 1399 10:44

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