بررسی ویژگی های 57 جایگاه تأیید شده CRISPR در 29 سویه از باکتری اشرشیاکلی

رحمت آبادی, سید سهیل and نظافت, نوید and نگهداری پور, مانیکا and حاجی قهرمانی, نسیم and مروت, محمد حسین and قاسمی, یونس (1395) بررسی ویژگی های 57 جایگاه تأیید شده CRISPR در 29 سویه از باکتری اشرشیاکلی. Journal of basic microbiology ــ 56 . pp. 1-9. شاپا 1521-4028

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Official URL: https://onlinelibrary.wiley.com/journal/15214028


Studying the features of 57 confirmed CRISPR loci in 29 strains of Escherichia coli

English Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) system is a novel type of innate defense system in prokaryotes for destruction of exogenous elements. To gain further insight into behavior and organization of the system, the extensive analysis of the available sequenced genomes is necessary. The dynamic nature of CRISPR loci is possibly valuable for typing and relative analyses of strains and microbial population. There are a few orderly bioinformatics investigations about the structure of CRISPR sequences in the Escherichia coli strains. In this study, 57 CRISPR loci were selected from 32 Escherichia coli strains to investigate their structural characteristics and potential functions using bioinformatics tools. Our results showed that most strains contained several loci that mainly included conserved direct repeats, while the spacers were highly variable. Moreover, RNA analysis of the sequences indicated that all loci could form stable RNA secondary structures and showed homology mostly with phages compared to plasmids. Only three strains included cas genes around their loci.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سید سهیل رحمت آبادی
نویسنده :نوید نظافت
نویسنده :مانیکا نگهداری پور
نویسنده :نسیم حاجی قهرمانی
نویسنده :محمد حسین مروت
نویسنده مسئول :یونس قاسمی
Additional Information:Impact factor: 1.76 Indexing in: ISI, Scopus, PubMed/Medline
Uncontrolled Keywords:کریسپر، اشرشیاکولی، ساختار RNA
کلیدواژه ها (انگلیسی):Cas / CRISPR / Direct repeat / Escherichia coli / RNA structure
Subjects:QV pharmacology > QV 737 Pharmaceutical Biotechnology
Divisions:School of Pharmacy > Department of Pharmaceutical Biotechnology
ID Code:12447
Deposited By: دکتر نسیم حاج قهرمانی
Deposited On:19 Dec 1398 10:30
Last Modified:19 Dec 1398 10:30

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