سمیت انتخابی کریسین در میتوکندری جدا شده از کبد مدل موش صحرایی hcc

صیدی, عنایت اله and رحیم پور, زهرا and سلیمی, احمد and پوراحمد, جلال (1398) سمیت انتخابی کریسین در میتوکندری جدا شده از کبد مدل موش صحرایی hcc. Bioorganic and Medicinal Chemistry ــ 27 (24). p. 115163. شاپا 0968-0896

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Selective toxicity of chrysin on mitochondria isolated from liver of a HCC rat model

English Abstract

Flavonoids are natural compounds that show various biological effects, such as the anti-cancer effect. Chrysin is a flavonoid compound found in honey and propolis. Studies have shown that chrysin has anti-cancer activity due to induction of apoptosis signaling. In the present study, we examined the cytotoxic effect of chrysin against liver mitochondria obtained from the hepatocellular carcinoma (HCC) rat model. Diethylnitrosamine (DEN) and 2-acetylaminofluorene (2-AAF) was used for induction of HCC. Mitochondria were isolated from liver hepatocytes using differential centrifugation. Then, hepatocytes and mitochondria markers related to apoptosis signaling were investigated. Our finding indicated an increase in mitochondrial reactive oxygen species (ROS) generation, collapse in the mitochondrial membrane potential (MMP), swelling in mitochondria, and cytochrome c release (about 1.6 fold) after exposure of mitochondria obtained from the HCC rats group with chrysin (10, 20, and 40 µM) compared to the normal rats group. Furthermore, Chrysin was able to increase caspase-3 activity in the HCC rats group (about 2.4 fold) compared to the normal rats group. According to the results, we proposed that chrysin could be considered as a promising complementary therapeutic candidate for the treatment of HCC, but it requires a further in vivo and clinical studies.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :عنایت اله صیدی
نویسنده :زهرا رحیم پور
نویسنده :احمد سلیمی
نویسنده مسئول :جلال پوراحمد
Additional Information:Impact Factor: 2.802 Indexed in: ISI, Scopus, PubMed/Medline, Embase
Uncontrolled Keywords:کرایسین، آپوپتوز، سرطان کبد، میتوکندری، سلول های کبدی
کلیدواژه ها (انگلیسی):Chrysin, Apoptosis, Hepatocellular Carcinoma , Mitochondria, Hepatocytes
Subjects:QV pharmacology > QV 600 Toxicology
QV pharmacology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:12512
Deposited By: دکتر احمد سلیمی
Deposited On:23 Sep 1398 15:42
Last Modified:23 Sep 1398 15:42

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