اکینوکوکوس مولتی لوکولاریس و اکینوکوکوس گرانولوزوس در سگهای استان خراسان شمالی، شمال شرقی ایران، شناسایی با استفاده از مورفولوژی و خصوصیات ژنتیکی DNA میتوکندری

حیدری, زهرا and شربت خوری, میترا and موبدی, ایرج and میرهندی, حسین and نیک منش, بهرام and شریف دینی, میثم and محبعلی, مهدی and زارعی, ذبیح الله and ارزمانی, کوروش and کیا, عشرت بیگم (1398) اکینوکوکوس مولتی لوکولاریس و اکینوکوکوس گرانولوزوس در سگهای استان خراسان شمالی، شمال شرقی ایران، شناسایی با استفاده از مورفولوژی و خصوصیات ژنتیکی DNA میتوکندری. Parasites & Vectors ــ 12 (606). pp. 1-13. شاپا 3.031

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Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA

English Abstract

Background: Canids are definitive hosts of Echinococcus multilocularis and Echinococcus granulosus. This study aimed to survey these two Echinococcus species in canids of North-Khorasan Province, northeastern Iran, using morphological criteria and genetic characterization of mitochondrial DNA. Methods: The carcasses of 106 canids, namely 61 jackals (Canis aureus), 23 foxes (Vulpes vulpes), 19 dogs (Canis familiaris) and three wolves (Canis lupus) were collected from the study area in 2013–2014 and examined for Echinococcus species. Morphological features were assessed by microscopy of adult worms. For molecular characterization, DNA was extracted, mostly from the adult worms but also from eggs. DNA fragments of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) mitochondrial genes were amplified and sequenced. Sequences were aligned and compared with reference sequences. Intraspecific and interspecific diversity were calculated and phylogenetic analysis was performed. Results: Overall, 9.4% of the canids (eight jackals and two foxes) were found infected with E. multilocularis by molecular methods, of which seven cases were also confirmed using morphological description of the adult worms. Echinococcus granulosus was found in 6.6% of the canines (four dogs, two jackals and one wolf ) as determined by both molecular methods and adult cestode morphology. All E. granulosus isolates were identified as the G1 genotype. Comparative sequence analysis indicated 0–0.7% and 0% intraspecific divergence within E. granulosus isolates and 0% and 0–0.2% within E. multilocularis isolates for cox1 and nad1, respectively. Conclusions: This study revealed the presence of E. multilocularis and E. granulosus in canids of North-Khorasan Province of Iran. Jackals were found infected with both E. multilocularis and E. granulosus, but infection with the former species was higher.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :زهرا حیدری
نویسنده :میترا شربت خوری
نویسنده :ایرج موبدی
نویسنده :حسین میرهندی
نویسنده :بهرام نیک منش
نویسنده :میثم شریف دینی
نویسنده :مهدی محبعلی
نویسنده :ذبیح الله زارعی
نویسنده :کوروش ارزمانی
نویسنده مسئول :عشرت بیگم کیا
Additional Information:IF: 3.031 Indexed in: ISI, PubMed/Medline, Scopus, Embase, DOAJ
کلیدواژه ها (انگلیسی):Canine, Echinococcus granulosus, Echinococcus multilocularis, Mitochondrial genes, Morphology, Iran
Subjects:QX Parasitology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:12590
Deposited By: خانم زهرا حیدری سورشجانی
Deposited On:06 Nov 1398 14:39
Last Modified:06 Nov 1398 14:39

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