آگروستما گیتاگو فعالیت کاسپاز 3/7 را در پروماستیگوت های لیشمانیا ماژور افزایش می دهد

عنوان انگليسی

Agrostemma githago increases caspase 3/7 activity in Leishmania major promastigotes

خلاصه انگلیسی

Introduction and objectives: Acquired resistance to anti-leishmanial drugs are increasing worldwide. Anti-leishmanial effect of Agrostemma githago seed extract on Leishmania major promastigotes has already been shown in our laboratory. This study was conducted to assess the induction of apoptosis in Leishmania major promastigotes after treatment with Agrostemma githago seed extract. Methods: The activity of caspase 3/7 as the key executioners of apoptosis were measured using the Caspase-Glo 3/7 Assay kit. Following treatment with EC50 dose of Agrostemma githago (0.4 mg/ml), 1:1 ratio of Caspase-Glo 3/7 reagent and Leishmania major promastigotes suspension containing 5 × 106 cells were added into white-walled 96 well plates and incubated at room temperature for 3 hours. The cleavage of tetrapeptide sequence DEVD by caspase enzymes was measured at 570 nm emission using plate-reading luminometer (Synergy HT, BioTek). Data are reported as the relative caspase activity in comparison to control group. Results: Our data showed that the activity of caspase 3 and 7 were increased in Agrostemma githago received group in comparison to untreated control. Discussion and Conclusion: Aqueous extract of Agrostemma githago seed induced apoptosis in Leishmania major promastigotes via increasing caspases 3 and 7 enzymes activity.

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