title

مقاومت به سطوح بالای آمینوگلیکوزیدها و آمپیسیلین در میان ایزوله های انتروکوکو جدا شده از نمونه های بالینی در یک بیمارستان ریفرال در ایران

موسوی, سیدحسین and پیری دوگاهه, هادی and محمدی قلعه بین, بهنام and تیمورپور, رقیه and ملکی, دادرس and خادمی, فرزاد and ارزنلو, محسن (1399) مقاومت به سطوح بالای آمینوگلیکوزیدها و آمپیسیلین در میان ایزوله های انتروکوکو جدا شده از نمونه های بالینی در یک بیمارستان ریفرال در ایران. Iranian Journal of Microbiology ــ 12 (4). pp. 319-324. شاپا 2008-3289

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Title

High-level resistance to aminoglycosides and ampicillin among clinical isolates of Enterococcus species in an Iranian referral hospital

English Abstract

Background and Objectives: Nowadays, high-level aminoglycosides and ampicillin resistant Enterococcus species are among the most common causes of nosocomial infections. The present study was conducted to determine the prevalence of high-level resistance to aminoglycosides and ampicillin among clinical isolates of Enterococcus species in Ardabil, Iran. Materials and Methods: In this cross–sectional study, a total of 111 Enterococcus species were collected from different clinical specimens between 2013 and 2015. Enterococcus species were identified using standard phenotypic and genotypic methods. BHI agar screen and agar dilution methods were used for detection of high-level gentamicin and streptomycin resistance (HLGR and HLSR) and minimal inhibitory concentration (MIC) of ampicillin, respectively.Results: Of 111 clinical isolates, 59 (53.2%) and 25 (22.5%) isolates were E. faecalis and E. faecium, respectively, based on the PCR results. Totally, 60.3% and 56.7% of isolates were HLGR and HLSR, respectively, as well as 51.35% were HLGR plus HLSR. Among HLGR isolates, 36 (61.01%), 18 (72%) and 13 (48.14%) were E. faecium, E. faecalis and non-faecalis non-faecium species, respectively. Among HLSR isolates, 33 (55.93%), 16 (64%) and 14 (51.85%) were E. faecalis, E. fae-cium and non-faecalis non-faecium species, respectively. All HLGR isolates contained aac(6 ́)Ie-aph(2′′)Ia gene. Overall, the prevalence of high-level ampicillin resistance among Enterococcus species was 17.1%. For E. faecalis, E. faecium and non-faecalis non-faecium species, ampicillin resistance rates were as follows: 11 (40.74%), 7 (28%) and 1 (1.69%), respec-tively. For aminoglycoside antibiotics, the resistance rate was significantly higher in E. faecium isolates and for ampicillin it was higher in E. faecalis isolates. Conclusion: The frequency of high-level aminoglycoside resistant enterococcal isolates in our hospital was high and signif-icant ampicillin resistance was noticed. This would require routine testing of enterococcal isolates for HLAR and ampicillin susceptibility.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سیدحسین موسوی
نویسنده :هادی پیری دوگاهه
نویسنده :بهنام محمدی قلعه بین
نویسنده :رقیه تیمورپور
نویسنده :دادرس ملکی
نویسنده :فرزاد خادمی
نویسنده مسئول :محسن ارزنلو
Additional Information:Indexed in: Scopus, PubMed/PMC, ESCI, Embase
کلیدواژه ها (انگلیسی):Enterococcus faecalis; Enterococcus faecium; High-level resistance; Gentamicin; Streptomycin; Ampicillin
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of physiology
Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:13251
Deposited By: Dr Mohsen Arzanlou
Deposited On:14 May 1399 11:24
Last Modified:29 Sep 1399 09:46

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