بررسی ارتباط میان چندریختی های موجود در جایگاه اتصال میکروRNAها در ژن MTHFR و زایمان زودرس در استان اردبیل

عنوان انگليسی

Investigation of the association between SNPs in the microRNAs binding site in the MTHFR gene and preterm labor in Ardebil province

خلاصه انگلیسی

Background: Preterm labor is the mostcommon cause of neonatal mortality, while infant mortality rates are acriterion for assessing the quality and comparisons of health systems incommunities. Evidence suggests that MTHFR polymorphism is currently involved inearly delivery. Methods: In this case-control study, 50 pregnant womenwith preterm labor (less than 37 weeks) as case group and 50 pregnant womenwith a delivery of more than 37 weeks were selected as control group. From eachindividual, 2.5 cc of blood was collected in EDTA tubes after extraction of DNAfrom the blood, the pcr reaction was performed and the results were analyzed onagarose gel. The polymorphism of pcr products was investigated by Revers DotBlot Technique. Data were analyzed by SPSS software usingt-test and Mann-Whitney test. Results: The prevalence of C677T polymorphism amongwomen with preterm delivery and control was 50% and 60%respectily. Forty-four percent of women with preterm labor and 60% of control group wereheterozygote for A1298C polymorphism and 50% of women with preterm deliverywere homozygous for this polymorphism, while in control group 40% of thehomozygotes were observed. The prevalence of A1298C polymorphism among womenwith preterm delivery and control was 80% and 70%respectily. 66% of women withpreterm delivery and 70% of control group were polymorphisms of A1298C geneheterozygote and 14% of women with preterm delivery were homozygous for thispolymorphism, while in the control group women did not have a consensus. Conclusion: In this study, there was a significant relationship between C677T andA1298C polymorphisms of MTHFR gene with preterm labor.

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