اثر عصاره هیدروالکلی کاپاریس اسپینوزا بر جزایر لانگرهانس جدا شده از پانکراس موش صحرایی

عنوان انگليسی

Effect of Caparis Spinosa Alcoholic Extract on the Isolated Rat Pancreatic Islets

خلاصه انگلیسی

Objective: From ancient time, plants were used to cure human diseases. Capparis spinosa L. belongs to the Capparaceae family and was reported to have some biological activities such as cytotoxic, anti-inflammatory, antimutagenic, antioxidant and anti-diabetic effect. It has very active chemical groups such as alkaloids, phenolic, flavonoids, tannins, and many other minerals. Phenol compounds have antioxidant activity and can scavenge free radicals so that plants containing these compounds have promissing effects for human health. Moreover, flower bud pickles of C.spinosa are traditionally used as a food supplement for treatment of diabetes. The aim of this study was to evaluate the effect of C.spinosa alcoholic extract on cellular viability and oxidative stress in rat pancreatic islets. Material and Method: In this experimental set up, the male rats were anesthetized and their pancreas were removed, then the islets of Langerhans were isolated and incubated in vitro for 24 hours. The islets were incubated with plant extract for 24 hours after which cellular viability and production of reactive oxygen species were measured. Results: The C.spinosa bud alcoholic extract markedly increased survival of the islet cells. This effect increased by increasing the doses of extract and greatest effects were observed in 103 and 104 μgmL-1 (p <0.001). Also, at mentioned concentrations, ROS production was reduced up to 37% (p <0.01) and 72 % (p <0.001) of control, respectively. Conclusion: Based on these results, we suggest that C.spinosa can be a promising candidate for prophylaxis and treatment in therapeutic strategies of diabetes.

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