title

ویتامین د و اریتروپویتین در مقابل آسیب ایسکمی-رپرفیوژن کلیه با بیان HSP70 , MiR-21 حفاظت می کند

گل محمدی, محمد قاسم and بنایی, شکوفه and نجاتی, کاظم and چینی فروش, میرمهدی (1399) ویتامین د و اریتروپویتین در مقابل آسیب ایسکمی-رپرفیوژن کلیه با بیان HSP70 , MiR-21 حفاظت می کند. Scientific Reports ــ . شاپا 2045-2322 (In Press)

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Official URL: https://www.nature.com/articles/s41598-020-78045-3...


Title

Vitamin D3 and erythropoietin protect against renal ischemia‑reperfusion injury via heat shock protein 70 and microRNA‑21 expression

English Abstract

Kidney ischemia reperfusion (IR) contributes to the development of acute kidney injury. The hypoxic conditions in ischemic damage lead to oxidative stress and apoptotic cell death. We investigated the effects of vitamin D3 (Vit D) and erythropoietin (EPO) on microRNA-21(miR-21) expression in renal IR. Wistar rats were divided into five groups including the control, vehicle + IR, Vit D + IR, EPO + IR, and Vit D + EPO + IR groups. The animals were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 h reperfusion. Vitamin D3 and EPO were administered prior to ischemia. After 24 h reperfusion, the kidney samples were collected for the detection of miR-21, heat shock protein 70 (hsp70) and caspase-3 expression levels. Kidney IR significantly increased the expression of miR-21, hsp70 and capase-3 and blood urea nitrogen (BUN)-Cr levels. Treatment with vitamin D3 and EPO significantly decreased the BUN-Cr levels and hsp70 and caspase-3 expression. Also, the co-administration of two drugs significantly increased miR-21 expression. It seems that vitamin D3 or EPO administration could protect the kidney against IR injury. However, vitamin D3 and EPO co-treatment was the most effective compared with the other treatment groups.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محمد قاسم گل محمدی
نویسنده مسئول :شکوفه بنایی
نویسنده :کاظم نجاتی
نویسنده :میرمهدی چینی فروش
Additional Information:IF: 3.998 Indexed in: ISI, Scopus, PubMed/Medline, DOAJ
کلیدواژه ها (انگلیسی):Erythropoietin, Renal ischemia-reperfusion, Vitamin D3, MiR-21, HSP70
Subjects:QT physiology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Pathology
Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
Faculty of Medicine > Department of Basic Sciences > Department of physiology
Vice Chancellor for Research and Technology > Pharmaceutical Sciences Research Center
ID Code:13728
Deposited By: Dr shokofeh banaei kivi
Deposited On:29 Sep 1399 08:52
Last Modified:29 Sep 1399 08:52

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