واحدی, پرویز ، حسین زاده گان, حسن ، برازوان, بلال ، روشنگر, لیلا ، شفایی, هاجر ، سلیم نژاد, رامین (1399) درمان نقص غضروفی توسط سونوگرافی پالسی با شدت کم در مدل گوسفند. Cell and Tissue Banking ــ . شاپا 1389-9333 (در حال چاپ )
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آدرس اینترنتی رسمی : https://link.springer.com/article/10.1007/s10561-0...
عنوان انگليسی
Treatment of cartilage defects by Low-intensity pulsed ultrasound in a sheep model
خلاصه انگلیسی
Aim of this study was to evaluate effects of Low-intensity pulsed ultrasound on repair of articular cartilage defects. Low-intensity pulsed ultrasound (Lipus) can induce the differentiation and activation of chondrocytes. This study was designed to evaluate the effect of Lipus on articular cartilage defects in a sheep. Eight sheep were divided in to two groups. The animals received bilateraly, articular cartilage defects 4 mm in diameter and 2 mm in deep on the patellar groove and experimental groups were treated with intensity 200 mW/cm², 20 min/day with low-intensity pulsed ultrasound for 2 month. Then both knee joints underwent surgery for remove of formed tissue sample from defects.The samples were evaluated by Quantitative real-time polymerase chain reaction (qRT-PCR), Safranin-o staining, Immunofluorescence Staining and Morphological characterization. The best and worst sample per group according to Macroscopic and micriscopic scoring were icentified. The results showed that the operated groups with-Lipus-treatment and without-Lipus treatment had considered statistically significant. Gross photography revealed that the defects in experimental groups were filled with proliferative tissue, while in control groups, a thin layer of proliferative tissue was formed in defects. qRT-PCR results showed the expression of coll2, sox9, aggrecan and Osteocalcin in experimental groups. Intense safranin-O staining show the formation cartilage tissue in ultrasound treated group, while loose safranin-o-staining were observed at the control groups. Immunofluorescence staining showed the type 2 Collagen protein expression. We suggest that low-intensity pulsed ultrasound provide the mechanistic basis force for articular cartilage repair and effective treatment modality for improving of articular cartilage defects.
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده مسئول : | پرویز واحدی |
نویسنده : | حسن حسین زاده گان |
نویسنده : | بلال برازوان |
نویسنده : | لیلا روشنگر |
نویسنده : | هاجر شفایی |
نویسنده : | رامین سلیم نژاد |
ضریب تاثیر و نمایه مجلات: | IF: 1.149 Indexed in: ISI, PubMed/Medline, Scopus |
کلیدواژه ها (انگلیسی): | Articular cartilage treatment; Cartilage defects; Low intensity pulsed ultrasound; Osteoarthritis. |
موضوعات : | QS آناتومی انسان WE سیستم عضلانی اسکلتی |
بخش های دانشگاهی : | دانشكده پزشكي > گروه علوم پایه > بخش علوم تشريح |
کد شناسایی : | 14002 |
ارائه شده توسط : | دکتر رامین سلیم نژاد |
ارائه شده در تاریخ : | 27 بهمن 1399 10:34 |
آخرین تغییر : | 27 بهمن 1399 10:34 |
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