بررسی اثرات مهاری برخی از گیاهان دارویی روی تولید نیتریک اکساید در سلول های PC12 استرس دیده

پارسا خانکندی, حامد and بهزاد, سحر and صحرانورد, شمیم and رضوانی, مینا and تدریس حسنی, نغمه (1398) بررسی اثرات مهاری برخی از گیاهان دارویی روی تولید نیتریک اکساید در سلول های PC12 استرس دیده. Pharmaceutical Sciences ــ 25 (2). pp. 132-137. شاپا 1735-403X

Text - Published Version

Official URL: https://ps.tbzmed.ac.ir/Article/Pharm-31731


Investigation of Some Medicinal Plants Inhibitory Effect on NO Production in Oxidative Stressed PC12 Cells

English Abstract

Background: Nitric oxide and reactive nitrogen species play an important role in various pathological conditions like cancer, inflammation and neurodegeneration. As plants and natural compounds have a great potency of discovering lead compounds which might affect NO production during inflammation and various pathologies, we examined the effects of three medicinal plants native to Iran, on NO production during oxidative stress in PC12 cells. Methods: In this study, cell death and NO levels were measured by MTT and by Griess assay, respectively. Oxidative stress was induced by hydrogen peroxide and extracts of Astragalus jolderensis, Convolvulus commutatus and Salvia multicaulis were used as pretreatment in oxidative stressed PC12 cells. Results: A. jolderensis extract significantly suppressed NO production in 150 and 200 μg/ml concentrations and C. commutatus extract in all concentration inhibited NO production in stressed PC12 cells. In addition, the extract of S. multicaulis inhibited NO production during stress at all concentrations above 50 μg/ml. Besides, the extract of S. multicaulis showed protective effect at lower doses in stressed cells. Conclusion: According to the results, S. multicaulis inhibited NO production and protected cells from oxidative stress. Hence, S. multicaulis is a good candidate for further in vitro and in vivo investigations. A. jolderensis and C. commutatus also suppressed NO production during stress. Therefore, they could be noticed in experiments that centralize on the inhibition of NO production and drug discovery studies in the field of neurodegenerative and chronic inflammatory diseases.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :حامد پارسا خانکندی
نویسنده :سحر بهزاد
نویسنده مسئول :شمیم صحرانورد
نویسنده :مینا رضوانی
نویسنده :نغمه تدریس حسنی
Additional Information:Indexed in: Scopus, ESCI, Embase
کلیدواژه ها (انگلیسی):Nitric Oxide, PC12, Astragalus jolderensis, Convolvulus commutatus, Salvia multicaulis, Griess assay
Subjects:QV pharmacology > QV 752 Pharmacognosy
QV pharmacology > QV 766 Plants, Medicinal
Divisions:School of Pharmacy > Department of Pharmacognosy
ID Code:14046
Deposited By: Dr Hamed Parsa Khankandi
Deposited On:12 Dec 1399 09:09
Last Modified:12 Dec 1399 09:09

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