یوسفی اوروند, ارشید ، مشکات, زهرا ، خادمی, فرزاد ، تفقدی, محسن (1400) ایمنی زایی فیوژن پروتئین HspX / EsxS مایکوباکتریوم توبرکلوزیس همراه با نانوادجوانت های ISCOMATRIX و PLUSCOM پس از تجویز زیر جلدی در مدل حیوانی. Microbial Pathogenesis ــ 154 (May). ص. 104842. شاپا 0882-4010
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عنوان انگليسی
Immunogenicity of HspX/EsxS fusion protein of Mycobacterium tuberculosis along with ISCOMATRIX and PLUSCOM nano-adjuvants after subcutaneous administration in animal model
خلاصه انگلیسی
Background: Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tuberculosis), is one of the most common and dangerous infectious diseases in the world. Despite vaccination with BCG, it is still considered as a major health problem. Therefore, design and production of an effective novel vaccine against TB is necessary. Our aim was to evaluate immunogenicity of HspX/EsxS fusion protein of M. tuberculosis along with ISCOMATRIX, PLUSCOM nano-adjuvants and MPLA through the subcutaneous route in mice model. Methods: HspX/EsxS fused protein of M. tuberculosis was cloned, expressed and purified in the prokaryotic system. ISCOMATRIX and PLUSCOM nano-adjuvants were prepared by film hydration method. Subcutaneous immunization of BALB/c mice was performed by different formulations. IFN-γ, IL-4, IL-17 and TGF-β cytokines levels as well as serum IgG1, IgG2a. Results: Our results showed that subcutaneous administration of mice with HspX/EsxS along with three adjuvants, ISCOMATRIX, PLUSCOM and MPLA increased immunogenicity of multi-stage fusion protein of M. tuberculosis. Additionally, HspX/EsxS protein + ISCOMATRIX or + PLUSCOM nano-adjuvants induced stronger Th1, IgG2a and IgG1 immune responses compared to MPLA adjuvant. Totally, HspX/EsxS/ISCOMATRIX/MPLA, HspX/EsxS/PLUSCOM/MPLA and two BCG booster groups could significantly induce higher Th1 and IgG2a immune responses. Conclusion: With regard to ability of ISCOMATRIX, PLUSCOM and MPLA adjuvants to increase immunogenicity of HspX/EsxS protein through induction of IFN-γ and IgG2a immune responses, it seems that these adjuvants and especially ISCOMATRIX and PLUSCOM, could also improve BCG efficacy as a BCG booster.
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده اول : | ارشید یوسفی اوروند |
نویسنده : | زهرا مشکات |
نویسنده : | فرزاد خادمی |
نویسنده مسئول : | محسن تفقدی |
ضریب تاثیر و نمایه مجلات: | IF: 2.914 Indexed in: ISI, PubMed/Medline, Scopus, Embase |
کلیدواژه ها (انگلیسی): | Mycobacterium tuberculosis , HspX/EsxS fusion protein , ,ISCOMATRIX , PLUSCOM , MPLA |
موضوعات : | QW میکروب شناسی و ایمنی شناسی |
بخش های دانشگاهی : | دانشكده پزشكي > گروه علوم پایه > بخش میکروبیولوژی |
کد شناسایی : | 14163 |
ارائه شده توسط : | دکتر فرزاد خادمی |
ارائه شده در تاریخ : | 07 تیر 1400 11:37 |
آخرین تغییر : | 07 تیر 1400 11:37 |
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