عصاره مرزنجوش موجب القای آپوپتوز در رده سلولی لوسمیک Molt-4 می شود

عنوان انگليسی

Origanum vulgare extract induces apoptosis in Molt-4 leukemic cell line

خلاصه انگلیسی

BACKGROUND: Origanum vulgare is a perennial plant which been widely used in traditional medicine, and its anti-oxidant properties have been reported. Acute lymphocytic leukemia is characterized by the accumulation of a large number of lymphoblastic cells. OBJECTIVES: The purpose of this paper is to investigate the effect of Origanum vulgare extract as plant with high anti-oxidant components on induction of cell death in Molt-4 cell line. METHODS: MTT assay was performed to determine the effect of O.vulgare extract on Molt-4 cells viability and the amount of 50% inhibitory concentration (IC50) was calculated. Changes in the expression of BAX and BCL-2 genes as involved in apoptosis and Nrf2 gene as transcription factor of anti-oxidant genes in O.vulgare extract treated Molt-4 cells were measured with Real Time PCR. Treated Molt-4 cells were used to determine the stages of early and late apoptosis, and necrosis using acridine orange/ethidium bromide double staining. REULTS: The results suggest survival inhibition and induction of apoptosis in Molt-4 cells treated with O.vulgare extract. Against Bax and Nrf2 genes expression, the expression of Bcl-2 gene have been reduced in Molt-4 cells following1/5 IC50 concentration of O. vulgare extract treatment. CONCLUSIONS: Given the oxidant drugs used in ALL treatment, and increased levels of oxidative stress in leukemic cells, induction of apoptosis by an anti-oxidant plant extract seems to be a promising way in leukemia treatment.

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