کدام جهش های نقطه ای در DNA ژیراز و توپوایزومراز IV در مقاومت ایزوله های بالینی سودوموناس آئروژینوزا در برابر سیپروفلوکساسین در اردبیل موثر هستند؟

خلاصه فارسی

با سلام خواهشمندم مشخصات مجله را بطور کامل بارگذاری فرمایید با تشکر اداره علم سنجی

عنوان انگليسی

Which missense mutations associated with DNA gyrase and topoisomerase IV are involved in Pseudomonas aeruginosa clinical isolates resistance to ciprofloxacin in Ardabil?

خلاصه انگلیسی

Ciprofloxacin is one of the most effective antibiotics against Pseudomonas aeruginosa (P. aeruginosa) infections. However, the prevalence of ciprofloxacin-resistant P. aeruginosa isolates is increasing in the world. In the current study, we assessed ciprofloxacin resistance rate, the mechanism of resistance to ciprofloxacin and also clonal relationships among ciprofloxacin-resistant P. aeruginosa in Ardabil. Applying phenotypic and genotypic methods, a total of 84 isolates of P. aeruginosa were collected from clinical specimens in Ardabil hospitals. P. aeruginosa resistance to ciprofloxacin antibiotic was evaluated using disk diffusion test. Genomic DNA was extracted using boiling method. Amplification of the gyrA, gyrB, parC and parE genes and analysis of gene mutations and amino acid alterations were performed through polymerase chain reaction (PCR) and sequencing assays. Finally, genetic diversity of ciprofloxacin-resistant P. aeruginosa isolates was determined by the enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) typing method. P. aeruginosa resistance to ciprofloxacin was 48.8%. The most prevalent missense mutations in the QRDR regions of target genes were as follows: Thr83Ile and Asp87Asn in the GyrA subunit, and Ser87Leu and Ser87Trp in the ParC subunit. There were no amino acid alterations in the GyrB and ParE subunits in ciprofloxacin-resistant P. aeruginosa isolates. In addition, ciprofloxacin-resistant P. aeruginosa were divided into 34 ERIC-PCR types, out of which type 27 was the most prevalent genotype. P. aeruginosa resistance to ciprofloxacin was high in our study and missense mutations were involved in ciprofloxacin resistance. Hence, continuous monitoring of ciprofloxacin resistance along with genotyping of resistant isolates and identification of other mechanisms of resistance are recommended for a better management of P. aeruginosa infections.

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