سلولهای بنیادی عصبی بالغ درونزاد جمعیت کوچکی از سلولهای نگهدارنده لیبل را در مغز موش بالغ تشکیل میدهند

گل محمدی, محمد قاسم and آذری, حسن and مردانی, محمد and اسفندياري, ابراهيم and ریتز, رادنی لی (1387) سلولهای بنیادی عصبی بالغ درونزاد جمعیت کوچکی از سلولهای نگهدارنده لیبل را در مغز موش بالغ تشکیل میدهند. در: چهارمین کنگره بین المللی سلولهای بنیادی رویان, تهران.

Text - Published Version


Endogenous Adult Neural Stem Cells Comprise The Minority of Label Retaining Cells in the Adult Mouse Brain

English Abstract

Objective: Given rapidly dividing and slow cycling populations of neural precursors have been identified in the adult brain, with several lines of evidence suggesting the later population represents Neural Stem Cells (NSCs), we hypothesized that adult NSCs could be identified using Label Retaining Cell (LRC) approach. Materials and Methods: Adult CBA mice were injected with the mitotic marker bromodeoxyuridine (BrdU), every two hours for 48 hours. We compared the number of LRCs detected in a 400µm region of the PVR at increasing chase periods to the number of primary neurospheres and NSC-derived colonies (Large Colonies) that could be generated from the same region using Neurosphere Assay (NSA) and Neural Colony-Forming Cell Assay(N-CFCA). Results: While the prevalence of LRCs and neurospheres and overall colonies was equivalent at specific chase periods,the number of NSC-derived colonies remained reduced by at least two orders of magnitude for chase periods up to 7 months. Conclusion: Our results suggest that < 5% of LRCs are bona fide neural stem cells and highlights the pitfalls of employing this methodology to discern stem from progenitor cell populations.

Item Type:Conference or Workshop Item (Speech)
زبان سند : انگلیسی
نویسنده مسئول :محمد قاسم گل محمدی
Uncontrolled Keywords:سلولهای بنیادی عصبی-موش- سلولهای نگهدارنده لیبل
کلیدواژه ها (انگلیسی):neural stem cell-mouse-label retaining cell
Subjects:WL Nervous system
QS Human Anatomy
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:1448
Deposited By: Dr Mohammad Gasem Golmohammani
Deposited On:07 Apr 1389 00:45
Last Modified:08 Dec 1391 10:02

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