صفری راد, سمیه ، ارزنلو, محسن ، محمدشاهی, جعفر ، واعظ, حمید ، صاحبکار, امیر حسین ، خادمی, فرزاد (1400) شیوع و ویژگی های کلون پرخطر ST235 و متالو بتالاکتاماز مثبت سودوموناس آئروژینوزا در بیمارستان های اردبیل. Jundishapur Journal of Microbiology ــ 14 (3). e115819. شاپا 2008-3645
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آدرس اینترنتی رسمی : https://sites.kowsarpub.com/jjm/articles/115819.ht...
عنوان انگليسی
Prevalence and Characteristics of Metallo-beta-Lactamase-positive and High-risk Clone ST235 Pseudomonas aeruginosa at Ardabil Hospitals
خلاصه انگلیسی
Background: Carbapenems are the most commonly administered drugs for the treatment of multidrug-resistant Pseudomonas aeruginosa (MDR P. aeruginosa) infections. However, carbapenem-resistant P. aeruginosa is spreading rapidly and has led to a new threat to human health worldwide. Objectives: The current study aimed to determine the prevalence of imipenem-resistant P. aeruginosa, detect metallo-β-lactamase (MBL)-producer isolates, and evaluate their clonal relationships in strains isolated from patients referring to the hospitals of Ardabil city, Iran. Methods: The resistance rate to imipenem was evaluated using the disk diffusion method. Double-disk synergy test and PCR technique were used for phenotypic and genotypic screening of MBL-positive P. aeruginosa, respectively. Ultimately, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and multilocus sequence typing (MLST) methods were used for assessing clonal relatedness among the isolates. Results: The prevalence of imipenem-resistant P. aeruginosa strains was estimated at 57.1% (48 out of 84 isolates). In addition, 45 (93.7%) out of 48 imipenem-resistant P. aeruginosa isolates were phenotypically screened as MBL-positive, among which 16 (35.5%) and three (6.6%) isolates harbored blaIMP and blaVIM-1 genes, respectively. However, blaNDM, blaSIM-2, blaSPM, and blaGIM-1 genes were not detected in this study. MBL-producing P. aeruginosa strains were divided into 42 ERIC-PCR types. Based on the results of MLST, P. aeruginosa ST235 was the only identified sequence type. Conclusions: Our results revealed a high and alarming prevalence of imipenem-resistant and blaIMP-positive P. aeruginosa ST235 at Ardabil hospitals. Continuous monitoring is essential to control the further spread of this highly virulent and drug-resistant clone.
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده اول : | سمیه صفری راد |
نویسنده : | محسن ارزنلو |
نویسنده : | جعفر محمدشاهی |
نویسنده : | حمید واعظ |
نویسنده : | امیر حسین صاحبکار |
نویسنده مسئول : | فرزاد خادمی |
ضریب تاثیر و نمایه مجلات: | IF: 0.747 Indexed in: ISI, Scopus, Embase |
کلیدواژه ها (انگلیسی): | Pseudomonas aeruginosa, Imipenem, Drug Resistance, Metallo-Beta-beta-lactamase, Clonal Relation. |
موضوعات : | QW میکروب شناسی و ایمنی شناسی |
بخش های دانشگاهی : | دانشكده پزشكي > گروه علوم پایه > بخش میکروبیولوژی |
کد شناسایی : | 14525 |
ارائه شده توسط : | دکتر فرزاد خادمی |
ارائه شده در تاریخ : | 16 شهریور 1400 09:37 |
آخرین تغییر : | 23 اسفند 1401 10:53 |
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