اثرات سایتوتوکسیک نانوذره حاوی کورکومین متصل به فولیک اسید بر سلولهای سرطانی پستان و ارتباط آن با مهار فسفوریلاسیون STAT3

عنوان انگليسی

The Cytotoxicity Effect of Curcumin Loaded Folic Acid Conjugated-Nanoparticles on Breast Cancer Cells and Its Association with Inhibition of STAT3 Phosphorylation

خلاصه انگلیسی

Signal Transducer and Activator of Transcription 3 (STAT3) phosphorylation plays a key role in the transformation and proliferation of breast cancer cells. According to previous studies, STAT3 phosphorylation can be inhibited by curcumin in various cancer cells. Even so, its use is restricted due to low oral bioavailability. This study aimed to enhance curcumin solubility and bioavailability by loading it on Folic Acid-Selenium nanoparticles and determine their effect on STAT3 activation in breast cancer cell line. The synthesized nanoparticles were characterized by Fourier-transform infrared spectroscopy, Dynamic light scattering, and transmission electron microscope. MTT and western blotting methods were applied to determine the viability and protein level of MCF cell line. The size and zeta potential of nanoparticles were found to be about 130 nm and - 45 mV, respectively. The results indicated a reduction of 49.62 ± 1.85% (p\0.05) in the viability and 79% (P\0.0001) in the phospho-STAT3 (p-STAT3) level in the cell line tested. Curcumin-loaded folic acid conjugated nanoparticles could be an effective drug for breast cancer chemotherapy. Though, subsequent in vivo research is required to determine its efficiency and safety in clinical applications.

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