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لینالول سمیت سلولی ، استرس اکسیداتیو و آسیب های لیزوزومی / میتوکندری ناشی از بنزن را در لنفوسیت های انسانی معکوس می کند

سلیمی, احمد and خداپرست, فرزاد and بهلولی, شهاب and هاشمی دانش, نیلوفر and بقال دهخوارقانی, الهه and رضاقلی زاده, لطف اله (1400) لینالول سمیت سلولی ، استرس اکسیداتیو و آسیب های لیزوزومی / میتوکندری ناشی از بنزن را در لنفوسیت های انسانی معکوس می کند. Drug and Chemical Toxicology ــ . شاپا 1525-6014 (In Press)

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Title

Linalool reverses benzene-induced cytotoxicity, oxidative stress and lysosomal/mitochondrial damages in human lymphocytes

English Abstract

Benzene exposure results in bone marrow suppression, leading to a decrease in the number of circulating white blood cells, an increased risk of chronic lymphocytic leukemia, acute myeloid leukemia and aplastic anemia. Since the mechanism of induction of benzene toxicity is due to active metabolites through cytochrome p450 enzymes and production of reactive oxygen species (ROS), we hypothesized that natural compound such linalool with anti-inflammatory/antioxidant properties could be effective in reducing its toxicity. Lymphocytes isolated from healthy individuals were simultaneously cotreated with different concentrations of LIN (10, 25 and 50 µM) and benzene (50 µM) for 4 h at 37 °C. After incubation, the toxicity parameters such cytotoxicity, ROS formation, lysosomal membrane integrity, mitochondria membrane potential (ΔΨm) collapse, oxidized/reduced glutathione (GSH/GSSG) and malondialdehyde (MDA) were analyzed using biochemical and flow cytometry evaluations. Our data showed that benzene (50 µM) induced a significant increase in cytotoxicity, ROS formation, mitochondrial membrane potential (MMP) collapse, lipid peroxidation and oxidative stress while LIN with antioxidant potential reversed the toxic effects of benzene on isolated human lymphocytes. Our results suggest that LIN reduces and reverses benzene-induced cytotoxicity, oxidative stress and lysosomal/mitochondrial damages in human lymphocyte. This study demonstrated that cotreatment of LIN with benzene can reduce several parameters indicative of oxidative stress. As such, LIN could represent a potential therapeutic agent in reducing certain aspects of benzene-induced toxicity.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :احمد سلیمی
نویسنده :فرزاد خداپرست
نویسنده :شهاب بهلولی
نویسنده :نیلوفر هاشمی دانش
نویسنده :الهه بقال دهخوارقانی
نویسنده :لطف اله رضاقلی زاده
Additional Information:3.356 (2020) Impact Factor Indexed in: ISI, PubMed/Medline, Scopus, Embase
Uncontrolled Keywords:بنزن ، سمیت سلولی ، لنفوسیت های انسانی ، استرس اکسیداتیو ، لینالول
کلیدواژه ها (انگلیسی):Benzene, cytotoxicity, human lymphocytes, oxidative stress, linalool
Subjects:QV pharmacology > QV 600 Toxicology
QV pharmacology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
School of Pharmacy > Department of Toxicology
School of Pharmacy > Department of Pharmacology
ID Code:14560
Deposited By: Dr Ahmad Salimi
Deposited On:17 May 1400 08:04
Last Modified:17 May 1400 08:11

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