طراحی و ساخت نانوذرات نوین مغناطیسی چندکاره برای ژن رسانی به سلولهای سرطان سینه

عنوان انگليسی

Design and fabrication of novel multi-targeted magnetic nanoparticles for gene delivery to breast cancer cells

خلاصه انگلیسی

In this study, a novel multi-targeted magnetic nanoparticles (MTMNPs) are synthesized to investigate their potential application in DNA protection and targeted gene delivery to breast cancer cells. In the present study MTMNPs was prepared from polyethyleneimine coated Fe3O4 nanoparticles, polyethyleneimine-polylactic acid-polyethylene glycol-folic acid copolymer (PEI-PLA-PEG-FA) and DNA using double-emulsion solvent evaporation method. The EPPT peptide (Glu-Pro-Pro-Thr) is also incorporated into the MTMNPs as a targeting agent for overexpressed MUC-1 receptors on breast cancer cells. The DNA release ratio from the MTMNPs nanoparticles were significantly decreased with increasing the pH value and decreasing the temperature. The capability of the MTMNPs nanoparticles to protect the DNA from nuclease digestion and ultrasound damage were confirmed by gel electrophoresis assay. In vitro results demonstrated a novel MFMNPs nanoparticle remarkably reduced PEI-associated toxicity on MCF-7Â cells, compared to PEI/DNA complex. Moreover, the MFMNPs improved the efficiency of gene delivery in 10 serum medium by more than 2.98-fold, compared to PEI/DNA complex.

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