تمایز سلول های بنیادی پالپ دندان به موتور نورون فانکشنال: یک مطالعه در شرایط In vitro و ex vivo

درویشی, مرضیه and قاسمی حمیدآبادی, هاتف and نظم بجنوردی, مریم and سعیدنیا, سارا and ظهیری, ماریا and نیاپور, علی and علیزاده, رفیعه (1400) تمایز سلول های بنیادی پالپ دندان به موتور نورون فانکشنال: یک مطالعه در شرایط In vitro و ex vivo. Tissue and Cell ــ 72 (2021). pp. 1-11. شاپا 0040-8166

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Differentiation of human dental pulp stem cells into functional motor neuron: In vitro and ex vivo study

English Abstract

There are several therapeutic options for spinal cord injury (SCI), among these strategies stem cell therapy is a potential treatment. The stem cells based therapies have been investigating in acute phase of clinical trials for promoting spinal repair in humans through replacement of functional neuronal and glial cells. The aim of this study was to evaluate the differentiation of Human Dental Pulp Stem Cells (hDPSCs) into functional motor neuron like cells (MNLCs) and promote neuroregeneration by stimulating local neurogenesis in the adult spinal cord slice culture. The immunocytochemistry analysis demonstrated that hDPSCs were positive for mesenchymal stem cell markers (CD73, CD90 and CD105) and negative for the hematopoietic markers (CD34 and CD45). hDPSCs were induced to neurospheres (via implementing B27, EGF, and bFGF) and then neural stem cells (NSC). The NSC differentiated into MNLCs in two steps: first by Shh and RA and ; then with GDNF and BDNF administration. The NS and the NSC were assessed for Oct4, nestin, Nanog, Sox2 expression while the MNLCs were evaluated by ISLET1, Olig2, and HB9 genes. Our results showed that hDPSC can be differentiated into motor neuron phenotype with expression of the motor neuron genes. The functionality of MNLCs was demonstrated by FM1-43, intracellular calcium ion shift and co- culture with C2C12. We co-cultivated hDPSCs with adult rat spinal slices in vitro. Immunostaining and hoechst assay showed that hDPSCs were able to migrate, proliferate and integrate in both the anterolateral zone and the edges of the spinal slices.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مرضیه درویشی
نویسنده مسئول :هاتف قاسمی حمیدآبادی
نویسنده :مریم نظم بجنوردی
نویسنده :سارا سعیدنیا
نویسنده :ماریا ظهیری
نویسنده :علی نیاپور
نویسنده :رفیعه علیزاده
Additional Information:IF: 2.586 Indexed in: ISI ,PubMed/Medline, Scopus
Uncontrolled Keywords:همکشتی، سلولهای بنیادی پالپ دندان،کشت لایه بافتی، کشت ارگانوتایپ
کلیدواژه ها (انگلیسی):Co-cultivation, Human dental pulp stem cells (hDPSCs), Slice culture, Organotype culture
Subjects:QS Human Anatomy
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:14787
Deposited By: Dr Ali Niapour
Deposited On:24 Jul 1400 12:54
Last Modified:29 May 1401 10:12

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