اثر محافظتی سلولهای بنیادی مزانشیمال مشتق از ژله وارتون یند ناف روی فیبروز کلیوی ناشی از انسداد یکطرفه حالب در موش صحرایی

خردمند, علیرضا and هاشمی تبار, محمود and خردمند, پروین and ولی زاده, فرزین and کاوش, آرین (1399) اثر محافظتی سلولهای بنیادی مزانشیمال مشتق از ژله وارتون یند ناف روی فیبروز کلیوی ناشی از انسداد یکطرفه حالب در موش صحرایی. European Urology Open Science ــ 20 (20). pp. 48-53. شاپا 2666-1683

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Protective Effect of Wharton's Jelly-derived Mesenchymal Stem Cells on Renal Fibrosis in Rats with Unilateral Ureteral Obstruction

English Abstract

Abstract Background Renal failure is a global medical problem. The use of mesenchymal stem cells (MSCs) for preservation and regeneration of renal tissue in acute and chronic kidney diseases has recently been the focus of investigation. Objective To evaluate the protective effect of MSC injections in a rat model of kidney obstruction. Design, setting, and participants We assigned 15 male Wistar rats to three separate groups: the normal group underwent left nephrectomy; the control group underwent laparotomy and left ureter ligation followed by saline injection into the aorta; and the study group received MSCs injected into the aorta inferior to the left renal artery after ligation of the left ureter. Kidneys were harvested 4 wk later and renal parenchyma samples were used for trichrome staining and for expression analyses. Outcome measurements and statistical analysis The degree of kidney fibrosis was assessed on pathology. Real-time polymerase chain reaction was used to determine expression levels of VEGF, TNF-α, and E-cadherin, and ΔCT and ΔΔCT values were calculated. Data were analyzed using SPSS v19 with paired t tests and nonparametric independent-sample Kruskal-Wallis tests. Results and limitations Fibrosis in the study group decreased from grade 3 or 4 to grade 1. In the control group, TNF-α expression increased and E-cadherin expression decreased. After MSC injection into obstructed kidneys, TNF-α and E-cadherin expression levels decreased and increased respectively, reaching similar levels to those in the normal group. No correlation between tissue regeneration and VEGF levels was observed. More research is needed to focus on other angiogenic factors. Conclusions MSC injection could prevent fibrosis in obstructed rat kidney via alterations in TNF-α and E-cadherin expression. Patient summary We investigated the effect of stem cell injection in rats with kidney obstruction. The treatment led to changes in the levels of two biomarkers and reduced the amount of kidney fibrosis caused by kidney obstruction.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :علیرضا خردمند
نویسنده :محمود هاشمی تبار
نویسنده :پروین خردمند
نویسنده :فرزین ولی زاده
نویسنده مسئول :آرین کاوش
Additional Information:Indexed in:ISI, Scopus, PubMed, DOAJ
کلیدواژه ها (انگلیسی):Mesenchymal stem cells , Renal obstruction , Renal fibrosis Kidney failure
Subjects:WJ Urogenital System
Divisions:Faculty of Medicine > Department of Surgery
ID Code:14900
Deposited By: Dr Farzin Valizadeh
Deposited On:29 Aug 1400 17:50
Last Modified:01 Sep 1400 12:53

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