اثر حفاظتی ساپونین بر DNA فرقمانتاسیون اسپرم موشهای درمان شده با سیکلوفسفامید

تیمار, مژگان and بنایی, شکوفه and مهربان, زهرا and سلیم نژاد, رامین and گل محمدی, محمد قاسم (1400) اثر حفاظتی ساپونین بر DNA فرقمانتاسیون اسپرم موشهای درمان شده با سیکلوفسفامید. Andrologia ــ . شاپا 1439-0272 (In Press)

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Protective effect of saponin on sperm DNA fragmentation of mice treated with cyclophosphamide

English Abstract

Cyclophosphamide (CP) is a common chemotherapy drug with the testicular damage. The aim of this study was to investigate the effect of saponin (SP) on the toxicity of CP in the male reproductive system. Following an experimental pilot study for determining SP dose, 40 male mice (32 ±3g) were divided into 5 groups (n =8): control, sham (normal saline 0.2 ml/day), CP (15 mg/kg/week, intraperitoneally), SP (2.5 mg/kg/day, intraperitoneally) and saponin group with cyclophosphamide (SP + CP). After treatment, left testis were removed for the measurement of malonedialdehyde (MDA) and total antioxidant capacity (TAC) levels and sperm DNA fragmentation was assessed by SDFA kit. In the CP group, a significant decrease in motility, viability, count, normal morphology and DNA fragmentation of sperm and TAC was observed, while in MDA level, a significant increase was observed compared to the control group (p <0.05). Attenuated sperm parameters in CP group improved significantly in SP + CP group (p <0.05). According to the findings of this study, SP was able to alter the reproductive toxicity of CP in NMRI mice and increase the antioxidant capacity of the testis

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مژگان تیمار
نویسنده :شکوفه بنایی
نویسنده مسئول :زهرا مهربان
نویسنده :رامین سلیم نژاد
نویسنده مسئول :محمد قاسم گل محمدی
Additional Information:IF: 2.775 Indexed in: ISI, Scopus, PubMed/Medline, Embase
کلیدواژه ها (انگلیسی):Saponin, Cyclophosphamide, Sperm, Testis, DNA fragmentation
Subjects:QS Human Anatomy
QT physiology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:14955
Deposited By: Dr shokofeh banaei kivi
Deposited On:13 Sep 1400 12:44
Last Modified:04 Oct 1400 07:47

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