بررسی ماکروفاژهای همراه تومور در سرطان پستان انسان با روش فلوسایتومتری

عنوان انگليسی

Analysis of Tumor Associated Macrophages in human breast cancer by Flocytometry

خلاصه انگلیسی

Tumor infiltrating leukocytes are one of the significant parameters in evaluating the immunostatus of body defence response against tumor cells. Macrophages are known to play an important role in immune response against tumors.In this research we studied the Tumor Associated Macrophages(TAM)in 17 patients whom suffered from breast cancer by flow cytometry.Thirteen of these patients had invasive ductal carcinoma, two invasive lobular carcinoma, one intraductal carcinoma and one atypic medullary carcinoma according to the pathological reports.In intratumor studies its found that the infiltration of CD45 cells(Leukocytes) were 17.13% of tumor cell suspension(in the case of invasive ductal carcinoma this value was16.4%).Macrophages with CD45/CD14 phenotype were 2.45%of tumor cell suspensions.These cells were 25.87%of infiltrated leukocytes(CD45 cells).Also we used CD16 and HLA-DR markers for recoginazing the activated macrophages.Macrophages with CD16/CD14 phenotype were 1.52% and macrophages with HLA-DR/CD14phenotype were 1.48% of tumor cell suspensions.Also CD16/CD14 cells and HLA-DR/CD14 cells were countes in ratio to CD45 cells.CD16/CD14 cells were 15.77% and HLA-DR/CD14 cells were 13.81%.In this study we could not found a significant correlation between infiltration of macrophages with age of patients and with the size of tumors.

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