title

بررسی ماکروفاژهای همراه تومور در سرطان پستان انسان با روش فلوسایتومتری

امانی, داور and حسن, زهیر محمد (1374) بررسی ماکروفاژهای همراه تومور در سرطان پستان انسان با روش فلوسایتومتری. در: سومین کنگره سراسری بیوشیمی و علوم آزمایشگاهی ایران, 8-11 مهرماه 1374, ایران -تبریز.

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Title

Analysis of Tumor Associated Macrophages in human breast cancer by Flocytometry

English Abstract

Tumor infiltrating leukocytes are one of the significant parameters in evaluating the immunostatus of body defence response against tumor cells. Macrophages are known to play an important role in immune response against tumors.In this research we studied the Tumor Associated Macrophages(TAM)in 17 patients whom suffered from breast cancer by flow cytometry.Thirteen of these patients had invasive ductal carcinoma, two invasive lobular carcinoma, one intraductal carcinoma and one atypic medullary carcinoma according to the pathological reports.In intratumor studies its found that the infiltration of CD45 cells(Leukocytes) were 17.13% of tumor cell suspension(in the case of invasive ductal carcinoma this value was16.4%).Macrophages with CD45/CD14 phenotype were 2.45%of tumor cell suspensions.These cells were 25.87%of infiltrated leukocytes(CD45 cells).Also we used CD16 and HLA-DR markers for recoginazing the activated macrophages.Macrophages with CD16/CD14 phenotype were 1.52% and macrophages with HLA-DR/CD14phenotype were 1.48% of tumor cell suspensions.Also CD16/CD14 cells and HLA-DR/CD14 cells were countes in ratio to CD45 cells.CD16/CD14 cells were 15.77% and HLA-DR/CD14 cells were 13.81%.In this study we could not found a significant correlation between infiltration of macrophages with age of patients and with the size of tumors.

Item Type:Conference or Workshop Item (Speech)
زبان سند : انگلیسی
نویسنده مسئول :داور امانی
Uncontrolled Keywords:ماکروفاژهای همراه تومور ، سرطان پستان ، فلوسایتومتری
کلیدواژه ها (انگلیسی):TUMOR Associated Macrophages , Breast Cancer , Flow cytometry
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Immunology
ID Code:1540
Deposited By: Dr Davar Amani
Deposited On:12 May 1389 03:29
Last Modified:07 Jul 1393 05:16

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