امانی, داور and حسن, زهیر محمد (1377) تاثیر تیمار آنزیمی روی مارکر های سطحی سلولهای تک هسته خون محیطی. در: چهارمین کنگره بین المللی ایمنولوزی و آلرژی ایران, 23-25 اردیبهشت, ایران- اصفهان.
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Title
The effects of enzymatic treatment on mononuclear cell (MNC) surface markers
English Abstract
Enzymatic digestion is common method for isolation of cells from tissues.when we use this method the first question is :what is the effect of enzyme on cell surface markers?for this resason we examine the effect of collagenase and DNAase on cell surface markers.These two enzymes are commonly used in researches.We studied CD45,CD14,and HLA-DR in this investigation.In this study we use flow cytometry methods and Fluorescein Isothiocyanat(FITC and R-Phycoerythrin(R-PE) conjucated monoclonal antibodies. our results showed that enzymatic treatment has no effect on cell viability(p>0.05).Also enzymatic treatment decreased the levels of CD45, CD16,CD14 and HLA-DR markers but this decreasing were not statically significant.The maximum decreasing were seen in the case of CD14 marker expression.
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| زبان سند : | انگلیسی |
| نویسنده مسئول : | داور امانی |
| کلیدواژه ها (انگلیسی): | CD markers, Enzymatic treatment , mononuclear cells(MNC |
| Subjects: | QW Microbiology and Immunology |
| Divisions: | Faculty of Medicine > Department of Basic Sciences > Department of Immunology |
| ID Code: | 1543 |
| Deposited By: | Dr Davar Amani |
| Deposited On: | 12 May 1389 05:24 |
| Last Modified: | 07 Jul 1393 05:15 |
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